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Browsing Theses & Dissertations by Author "Acevedo Acevedo, Débora"
Acevedo Acevedo, Débora; Meléndez, Enrique; College of Arts and Sciences - Sciences; Cortés Figueroa, José E.; López, Gustavo; Department of Chemistry; Banejee, Jayanta
Molybdenocene dichloride like some other metallocene dihalides Cp2MX2 (M = Ti, V, Nb, Mo and X = halides) have shown high antiproliferative properties against a wide range of murine and human tumors. They also exhibit less toxic side effects than platinum antitumor agents. In this work we present the synthesis of four complexes of molybdenocene at physiological pH in aqueous medium. The complexes [(η5-C5H5)2Mo(L)]Cl (L=6-mercaptopurine , 6-mercaptopurine ribose, (-)-2-amino-6-mercaptopurine and (-)-2-amino-6-mercaptopurine ribose) were characterized by FT-IR, NMR, MS and elemental analysis. The synthesized, characterized and purified complexes were interacted with Human Serum Albumin (HSA), a 66.5 kDa protein with an impressive array of binding sites capable of binding a wide variety of ligands. The interaction between HSA with Cp2MoCl2 and [Cp2Mo(L)]Cl complexes was monitored by UV-Vis from 200 – 600 nm observing no new bands or isosbestic point and by NMR at 25 and 37ºC. Such interactions were not observed unlike to what was hypothesized by Harding et al. Cytotoxic studies were also performed on the four new molybdenocene derivatives on HT-29 colon cancer and MCF-7 breast cancer cell lines. These studies were compared to those carried out for molybdenocene dichloride. Complexes that contained ribose showed to be less active than those without ribose but all complexes proved to have more cytotoxic activity than molybdenocene dichloride in HT-29 colon cancer. In MCF-7 breast cancer, the addition of these bidentate ligands without a ribose group, improves the cytotoxic activity when compared to Cp2MoCl2. On the other hand the complexes with the ribose groups showed to be not active in MCF-7 cell line.