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Browsing Theses & Dissertations by Author "Acevedo Chaparro, Jensen Jovan"
Acevedo Chaparro, Jensen Jovan; Serrano, Guillermo; College of Engineering; Ducoudray, Gladys O.; Resto Irizarry, Pedro J.; Department of Electrical and Computer Engineering; Pagán Lugo, Solangie
This work presents the design of a custom microelectronic fluorescence sensing system for rapid detection of Enterococcus faecalis, enterococcus, or total coliform bacteria using commercially available EPA-approved bio-detection assays such as Enterolert. Photodiodes are used as transducers to convert the fluorescence light emitted from the assay into an electrical current signal that is proportional to bacteria concentration. Detection time reduction is achieved by using a gradient detector integrated circuit to calculate the rate of change of the photodiode current instead of measuring its absolute value. Circuit simulations of the proposed system, designed in a 0.18 um UMC CMOS technology, resulted in bacteria detection in 3 hours or less, as opposed to 24 hours required by the Enterolert test alone.