Vélez-Robles, Yomar R.

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    Segregation of heat shock factor-1, heat shock protein-70 and signal transducer of activation of transcription 1 polymorphisms in normal and slick coated cattle and its potential associations with molecular breeding values and phenotypical traits
    (2018-05) Vélez-Robles, Yomar R.; Pagán-Morales, Melvin; College of Agricultural Sciences; Jiménez Cabán, Esbal; Sánchez Rodríguez, Héctor; Curbelo Rodríguez, Jaime; Latorre Acevedo, José R.; Department of Animal Science; Román Pérez, Rosa I.
    Heat stress (HS) in dairy cattle is one of the major problems that contribute to farmer’s financial losses in tropical and subtropical climates. Dairy cattle under HS have lower milk yields, deficiency in reproductive traits and immune depression. Genetic selection of animals that present better thermo-tolerance helps offset the adverse effect of HS. Previously, genes that can help mitigate heat stress have been identified. Among these is the prolactin receptor (PRLR), which determines the type of hair coat; the heat shock transcription factor-1 (HSF1), which increases the production of heat shock protein-70 (HSP70); HSP70, helping with cell protection against stress events; and the signal transducer of activation of transcription 1 (STAT1), involved in milk production and embryo survival. The objectives of this study were to assess the segregation of polymorphisms in the aforementioned genes and evaluate its associations with production traits and molecular breeding values (MBV) in slick (SL) and normal (WT) coat Holstein cows. Segregation of a previously reported ApaLI restriction fragment length polymorphism (RFLP) resulting of a thymine to cytosine transition (genotypes: TT, TC and CC) at intron 3 of HSF1 (rs#133931291) was found. 2 STAT1 polymorphisms located at exon 11 (rs#209286339; aminoacid change phenylalanine to serine) and intron 11 (rs#211030653), respectively, were identified to be segregating in linkage disequilibrium (haplotypes: CC, CTCG and TG). STAT1 haplotypes interacted with the HSF1 genotypes to affect the MBV of productive life (PL) (P = 0.0494). Significant interaction was also found between HSF1 genotypes and coat type for the same MBV (P = 0.0456). Out of 11 polymorphisms previously reported on the HSP70 promoter, 6 were segregated in the estudied Holstein population and arranged in 12 haplotypes. Ultimately, 4 haplotypes were used for the correspondent statistical analyses and a trend toward significance was found for the MBV of somatic cell score (SCS, P = 0.0779). For the same MBV, a trend toward significance interaction was observed for STAT1 haplotypes with coat type (P = 0.0809). That interaction was significant for adjusted SCS determined by the Dairy Herd Improvement Program (P = 0.0157). Therefore, novel double interactions affecting PL molecularly were observed in Holstein cattle either associated or independently of coat type, and STAT1 seems to be involved with SCS phenotypically.