Navarro Gutiérrez, Patricia D.
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Publication Open Access Larval stages (instars) of the coffee leafminer 𝘓𝘦𝘶𝘤𝘰𝘱𝘵𝘦𝘳𝘢 𝘤𝘰𝘧𝘧𝘦𝘦𝘭𝘭𝘢 (GuérinMèneville) (Lepidoptera: Lyonetiidae) and its synchronization with the parasitoid 𝘔𝘪𝘳𝘢𝘹 𝘪𝘯𝘴𝘶𝘭𝘢𝘳𝘪𝘴 Muesebeck (Hymenoptera: Braconidae) in Puerto Rico.(2007) Navarro Gutiérrez, Patricia D.; Gallardo Covas, Fernando; College of Agricultural Sciences; Segarra Carmona, Alejandro E.; Armstrong Ramírez, Arístides M.; Macchiavelli, Raúl E.; Department of Agro-Environmental Sciences; Franz, Nico M.The coffee leaf miner (CLM) 𝘓𝘦𝘶𝘤𝘰𝘱𝘵𝘦𝘳𝘢 𝘤𝘰𝘧𝘧𝘦𝘦𝘭𝘭𝘢 (Lepidoptera: Lyonetiidae) is the main pest of coffee in the western central region of Puerto Rico. 𝘔𝘪𝘳𝘢𝘹 𝘪𝘯𝘴𝘶𝘭𝘢𝘳𝘪𝘴 (Hymenoptera: Braconidae) is one of the principal parasitoids of this pest present on the island. Three main objectives were carried out in this study: 1) to determine the number of instars of the CLM larvae under laboratory conditions, 2) to determine the number of instars in field collected samples and 3) to identify the instar (s) of 𝘓. 𝘤𝘰𝘧𝘧𝘦𝘦𝘭𝘭𝘢 preferred by 𝘔. 𝘪𝘯𝘴𝘶𝘭𝘢𝘳𝘪𝘴 for its parasitation. Samples of leaves infested with CLM were obtained from the coffee growing zones in Puerto Rico. Colonies of 𝘓. 𝘤𝘰𝘧𝘧𝘦𝘦𝘭𝘭𝘢 were reared under laboratory conditions and the larval head capsule widths were measured across their widest point, and the results were plotted. Larvae from the field collected samples were measured and analyzed in the same way. Four larval instars were observed in both cases (laboratory and field). The preferred instar of 𝘓. 𝘤𝘰𝘧𝘧𝘦𝘦𝘭𝘭𝘢 by 𝘔. 𝘪𝘯𝘴𝘶𝘭𝘢𝘳𝘪𝘴 was determined under laboratory conditions. Coffee plants were oviposited by 𝘓. 𝘤𝘰𝘧𝘧𝘦𝘦𝘭𝘭𝘢 during 48 hours, and when each instar was estimated to be formed, 150 parasitoids were introduced during 48 hours for its parasitization. The selection coefficient revealed that 𝘔. 𝘪𝘯𝘴𝘶𝘭𝘢𝘳𝘪𝘴 parasitized 1ˢᵗ and 2ⁿᵈ instar with a 60% and 63% respectively. Instars 3ʳᵈ and 4ᵗʰ were not parasitized.