Luciano Rosario, Dianiris

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    Molecular evaluation of the genetic diversity of papaya (Carica papaya) in Puerto Rico
    (2017) Luciano Rosario, Dianiris; Siritunga, Dimuth; College of Arts and Sciences - Sciences; Cuevas, Hugo E.; Porch, Timothy; Rodriguez Minguela, Carlos; Department of Biology; Del Rio, Luis O.
    Native to Central America, papaya (Carica papaya) is one of the most cultivated fruit crops in tropical areas of the world. The commercial success of papaya is not only due to its high nutritional qualities but also due of its short generation time. Assessing the genetic diversity of papaya is an important aspect of conservation of this important plant genetic resources. However, knowledge on the genetic diversity of papaya in Puerto Rico is poorly understood. Therefore, 139 papaya accessions collected from all over Puerto Rico were evaluated using 23 Simple Sequence Repeat (SSR) markers and compared to 13 varieties from the USDA repository and 10 commercial varieties that served as references. A total of 214 alleles were identified having a mean observed heterozygosity (Ho) of 0.219. The Inbreeding coefficient (F) yielded a value of 0.565 and when evaluating the population structure of these accessions, 2 groups (k=2) were identified. An Unweighted Pair Group Method with Arithmetic Mean (UPGMA) dendogram showed no geographical organization within the unknown Puerto Rico samples. Moreover, Single Nucleotide Polymorphisms (SNPs) identification using Genotyping by Sequencing was also used to assess the genetic diversity of the same samples. We found a total of 4, 245 SNPs. A mean observed heterozygosity (Ho) of 0.226 and Inbreeding Coefficient (Gis) of 0.067 was recorded. In agreement with the SSR analyses, the population structure showed that the samples grouped in 2 clusters (k=2). Overall, this study contributes to the knowledge of papaya genetic diversity in the Caribbean region which will be useful for the conservation of papaya genetic resources.