Barreto Diaz, Mairan Alejandra

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    Graviola leaf extract toxicity to tongue squamous cell carcinomas
    (2022-05-12) Barreto Diaz, Mairan Alejandra; Cassé, Céline; College of Arts and Sciences - Sciences; López Moreno, Martha Laura; Diffoot Carlo, Nanette; Department of Chemistry; Coutin Rodicio, Sandra
    Annona Muricata (Graviola) is a tropical tree that is common in the Puerto Rican landscape. The anticancer properties of molecules extracted from different parts of the graviola tree have been reported in various cell lines. In the present work, we have prepared an ethanolic Graviola leaf extract (EGLE) from a local tree specimen and assessed its antiproliferative properties in an in vitro model of Head and Neck Cancers. We report the first study’s results to compare the bioactivity of a Graviola leaf ethanolic extract on human Squamous Cell Carcinoma cell lines-9 and -25 (SCC-9 and SCC-25) and non-transformed Human Gingival Fibroblasts (HGF-1). The present project aims at assessing the antiproliferative/anti-cancer properties of the chemical compounds extracted from Graviola leaves. It is divided into three major studies: CHAPTER 1 details the protocols for preparing a crude extract ethanolic Graviola leaf extract (EGLE) suitable for in vitro cell culture experiments. CHAPTER 2 run a comparative assessment of the antiproliferative properties of EGLE on Squamous Cell Carcinoma lines-9, line- 25 (SCC-9, SCC-25), and non-transformed Human Gingival Fibroblasts-1 (HGF-1) CHAPTER 3 analyzes the chemical composition of EGLE using qualitative and quantitative analytical methods.