Avilés Vázquez, Christian O.

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    Description of the prokaryotic community in the bivalve Lucina pectinata in marine wetlands of Cabo Rojo, Puerto Rico!
    (2011) Avilés Vázquez, Christian O.; López Garriga, Juan; College of Agricultural Sciences; Pérez Bolivar, Juan; Alameda Lozada, Myrna; Department of Crops and Agro-Environmental Sciences; Romero, Juan
    Lucina pectinata is a large bivalve that belongs to the family Lucinidae; it is generally found in marine wetlands of the Caribbean, deeply burrowed in black mud with high sulfide concentrations. L. pectinata prokaryotic community was extracted by the Percoll cushion method and its cell populations were analyzed through Flow Cytometry. Fluorescence and conventional microscopy were performed to validate the data obtained through FCM. After analyzing the data, bacteria populations with a length of, approximately, 3μm and 6μm were defined. Statistical analyses were performed to determine the existence of a proportional relationship within the cell sizes in the populations according to the bivalve sizes, and the number of cell populations in relationship with the bivalve sizes. A statistical analysis was also performed to determine if the use of one ctenidia or two had an effect on the amount of populations reported by FCM. The statistical data supports that the bacteria cell sizes and the total populations are independent of bivalve size and the number of ctenidias used. Furthermore, the data supports the hypothesis that the presented symbiotic pattern is characteristic to bivalves present in environments with relatively high concentrations of hydrogen sulfide and low concentrations of oxygen.! !