Bueno-Vera, Juliana A.

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    Electrochemical characterization of MC3T3-E1 cells cultured on TiAI and Ti6AI-4V alloys for biological implant applications
    (2012) Bueno-Vera, Juliana A.; Sundaram, Paul A.; College of Engineering; Díaz-Rivera, Rubén; Diffoot, Nanette; Department of Mechanical Engineering; Shafiq, Basir
    One manner of understanding the processes that occur at the implant surface/cell interface is to utilize the electrochemical response of this system as a consequence of protein adsorption, cell adhesion and cell growth on the implant surface. The current study required the design and manufacture of an electrolytic cell to measure the electrochemical response. During different periods of cell growth Electrochemical Impedance Spectroscopy (EIS) was used to study the behavior of MC3T3-E1cells at the interface of two Ti-based alloys (Ti-6Al-4V and TiAl). The cells were incubated on these Ti-based alloys during 4, 7 and 14 days in αMEM+FBS solution. EIS measurements were carried out at the open-circuit potential in a frequency range from 1 mHz to 100 kHz. The results show an increase in polarization resistance (Rp) on the surfaces with cells as a function of time. In Nyquist plots two or more semi-circles were observed for alloys with and without cells on the metallic surfaces, associated with the passive oxide film, adsorption of proteins and cell tissue on surfaces with the passage of time. From SEM images, an optimal cellular activity was seen, based on the normal polygonal morphology, with long and fine cytoplasmic prolongations of the cells on Ti-6Al-4V and TiAl. Similarly, mineralization nodules were observed on surfaces associated with osseogenetic process. Immunofluorescence analysis to detect the presence of collagen Type I showed an increase in the segregation of collagen as a function of time. From this study, it can be concluded that the Rp values reflect the amount of collagen secreted by the cells and EIS may be a viable technique to study protein content in the extracellular matrix.