Marrero-Pérez, Marcela G.

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    Association between bovine milk infrared temperature and bacteriological results using the PathoProof™ Mastitis Complete-16 Kit
    (2017) Marrero-Pérez, Marcela G.; Curbelo-Rodríguez, Jaime E.; College of Agricultural Sciences; Ortiz Colón, Dr. Guillermo; Sánchez Rodríguez, Dr. Héctor; Jiménez Cabán, Dr. Esbal; Department of Animal Science; González Toledo, Dr. Marggie
    Infrared temperature (IRT) of the milk through the milking unit short milk tube and its association with bacteriological results using the PathoProof™ Mastitis Complete-16 Kit (PtoPrf-16) was evaluated. In addition, the sensibility and specificity of CHROMagar™ Mastitis Kit (CHROM) were studied using the PtoPrf-16 results. Individual mammary quarters (n=137) with subclinical mastitis (determined by the California Mastitis Test) from 9 dairy herds in Puerto Rico were evaluated. Milk samples (10 mL in duplicates) were collected and stored in ice for subsequent evaluation. During the a.m. milking, IR images were collected 2 min post milking unit attachment in 30s intervals. Ambient temperature (AT) and relative humidity (RH) were recorded using HOBO-U23Prov2Data Loggers. Somatic cell count (SCC) determination and milk plate culturing were done using a DeLaval Cell Counter and CHROM, respectively. Duplicated milk samples were sent to the Lancaster DHIA Lab for molecular identification of mastitic pathogens using the PtoPrf-16. A PROC GLIMMIX was used to determine differences between IRT and SCC by bacteriological results (PtoPrf-16) and contingency tables with a Chi-Square test to determine specificity and sensitivity of CRHOM plates relative to molecular results. The SCC was converted to somatic cell score (SCS) and bacteriological results grouped in Gram-positive, Gram-negative, and No-detection for analysis evaluation. The mean SCS differed between Gram-positive and no-detection (P=0.022) but not between the remaining comparisons (P=0.05). The mean IRT of the short milking tubes was higher in the Gram-positive group relative to no-detection (P=0.052) with mean IRT±SEM of 33.48±0.20 and 32.86±0.29, respectively. No differences in IRT were observed between the remaining comparisons (P>0.05). Using the PtoPrf-16 as a comparative test tool, the CHROM presented an overall sensitivity and specificity of 70.5% and 52.17%, respectively. According to these findings, the use of IRT could be used as a discriminatory tool between subclinical mastitis quarters and justify more studies to better characterize these differences.