García Cortés, Carlos G.

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    Isolation and characterization of protein complexes of overexpressed human serum amyloid a from cytokines-induced HepG2 cells
    (2023-05-10) García Cortés, Carlos G.; Parés Matos, Elsie I.; College of Arts and Sciences - Sciences; Román Velázquez, Felix R.; Rodríguez Medina, José R.; Department of Chemistry; Perea Nieves, Cristina
    Background and Objective: Serum Amyloid A (SAA) is an apolipoprotein involved in various physiological processes, including acute-phase response, cholesterol metabolism, retinol transport, and tumor pathogenesis. However, its exact biochemical role in many diseases has yet to be fully understood. Therefore, this research aims to identify the protein-protein interactions of SAA in human cells and to predict its biochemical role based on newly discovered complexes. Methods: HepG2 cells were induced with IL-1β, IL-6, Lipopolysaccharide, and Retinol, and the expression levels of SAA were measured using an ELISA assay. Protein complexes associated with SAA were isolated using the Co-Immunoprecipitation technique, and LC/MS/MS was used for characterization. Results: SAA was successfully overexpressed, and ten proteins were characterized by LC/MS/MS that may form complexes with SAA. Based on scientific publications, three of these proteins are associated with SAA. Discussion and Conclusions: The results of this research support some already established roles of SAA and provide new information related to its protein-protein interactions that could be used to develop new therapeutic interventions and diagnostic tools for diseases associated with SAA.