Guachambala-Cando, Marcelino S.

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    Estudio de la herencia de los altos niveles de resistencia al tizòn bacteriano, causado por Xanthomonas axonopodis pv. phaseoli, en el frijol comùn (Phaseolus vulgaris L.)
    (2013) Guachambala-Cando, Marcelino S.; Zapata-Serrano, Mildred; College of Agricultural Sciences; Porch, Timothy; Beaver, James; Van Bloem, Skip; Department of Crops and Agro-Environmental Sciences; Vieta, Rene
    Common bacterial blight caused by the pathogen Xanthomonas axonopodis pv. phaseoli (Xap) is an important biotic factor limiting common bean (Phaseolus vulgaris L.) production. A few bean breeding lines such as VAX 6 exhibit a high level of resistance to a wide range of Xap strains representing different pathogenic races. In this study, the inheritance of high levels of resistance to common bacterial blight with the pathogenic strain Xap UPR 3353 was evaluated in a population derived from a cross between breeding lines PR0313-58, which has moderate levels of resistance and VAX6, which has high levels of resistance to common bacterial blight. The genetics of resistance to Xap 3353 was studied using generation means analysis and Chi-square tests in the F2 and F3 generations. SCAR markers SAP6, SU91 and SU91-CG11 were evaluated, to detect possible cosegregation with high levels of resistance. Common bacterial blight evaluations were conducted in the field and in a greenhouse on F2 plants and F2:3 lines. The results suggest that in this population two putative dominant genes confer high levels of resistance to bacterial blight caused by strain Xap UPR 3353. White and black bean lines that combine high levels of common bacterial blight resistance, heat tolerance and resistance to BGYMV and BCMV virus were identified in this study. The DNA was extracted from F2, F3 and F4 plants and subsequently diagnosed for the presence or absence of SU91 and SU91-CG11 SCAR markers. There was no consistent cosegregation with plants that showed high levels of resistance. Therefore, there is a need to identify molecular markers that can facilitate the selection of these dominant genes that confer high levels of bacterial blight resistance.