Amaro Berrocal, Camille

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    The influence of thermic stress on the marine yeast Debaryomyces hansenii
    (2022-12-19) Amaro Berrocal, Camille; Schizas, Nikolaos V.; College of Arts and Sciences - Sciences; Parthasarathy, Venkata; Rodríguez Minguela, Carlos M.; Weil, Ernesto F.; Department of Marine Sciences; Alfaro, Mónica
    Often organisms must acclimate and adjust their metabolism to better adapt to an ever-changing environment. Some environments can be considered to have normalized parameters, while others truly challenge the homeostatic state of many microorganisms, although under both life seems to thrive. This project will attempt to understand these adaptations using the marine yeast Debaryomyces hansenii as a model system. Debaryomyces hansenii is an ascomycete marine yeast initially isolated from a Swedish estuary. It has been shown to tolerate much higher sodium chloride (NaCl) concentrations than those found in seawater. This yeast can grow in the presence of heavy metals as well as in a wide pH range. Moreover, D. hansenii is known to increase population at temperatures ranging from 11oC to 37oC (24oC, optimal), a broader scope than that reported for the most commonly known yeasts. Temperature, in particular, has been known to alter the tolerance response of D. hansenii toward other stressors. For example, in combination with high temperatures (~34C), NaCl stimulated growth beyond the recorded maximum for either of the stressors individually. Also, a higher tolerance against multiple stressors was observed at lower temperatures. The most commonly known stress studies for Debaryomyces were often conducted under a single temperature to observe the isolated effect of the stressors in question. However, in this study, a series of experiments were developed to understand better the dynamic and assimilation of multiple stressors under different thermal parameters. Due to the wide genomic divergence of the Debaryomycetea group, a genome sequence was performed to identify the phylogenetic space it occupied. The result showed this hansenii specie (strain J6) to be a subspecies of Debaryomyces fabryi. A physiological assessment provided information on population growth and death rate caused by the synergistic effects of the multi stressors conditions.