Poueymirou Rodríguez, Zilma J.

Profile Picture

Filters

20061
Reset filters

Settings

Citations

Publication Search Results

Now showing 1 - 1 of 1
  • Thumbnail Image
    Publication
    Quantitative determination of caffeine in Puerto Rico coffees varieties by SPME and GC/MS alternative method
    (2006) Poueymirou Rodríguez, Zilma J.; De Jesús Echevarría, Maritza; College of Arts and Sciencies - Sciences; Laboy, Jorge; Monroig Inglés, Miguel; Department of Chemistry; González, Wigmar
    This investigation was focused in the development of an alternative method for the caffeine quantitative analysis in the ground coffee of the principal commercial species of coffee (Coffea arabica and Coffea canephora) and their respective most important varieties, cultivated at different altitudes in Puerto Rico. These are Borbón, Caturra, Limaní and a coffee Robusta. The techniques employed were Solid Phase Microextraction (SPME) and Gas Chromatography coupled to Mass Spectroscopy (GC/MS) using the Extracted Ion Mode of the ions 194 and 197m/z that correspond to the mass spectra father peak of the original ¹²C caffeine and the isotopically ¹³C caffeine employed as the standard. The caffeine content in the Arabica and Robusta species was the same as established in the literature. The ground coffee that contains the higher concentration of caffeine, independent of the cultivation altitude, was the Robusta variety with an average of 1.80%w/w by dry matter. The caffeine concentration changed in the Arabica coffee variety, with the change in cultivation altitude. In general the variety under this specie with the most caffeine content was the Bourbon variety (1.26%w/w). The SPME extraction technique offer precise data that is in close agreement to the data obtained using traditional analytical methods. The validation procedure for SPME/GC-MS is an alternative method for the qualitative and quantitative analysis of caffeine in ground coffee.