Vargas-Palacios, Ana G.

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    Estudio de la reacción al Virus del Mosaico Necrótico Común del Frijol (BCNMV) y la habilidad de fijación biológica del nitrógeno (FBN) en frijol tépari (Phaseolus acuatifolius A. Gray) e introgresión de la FBN al frijol común (Phaseolus vulgaris L.).
    (2016) Vargas-Palacios, Ana G.; Estévez-de Jensen, Consuelo; College of Agricultural Sciences; Porch, Timothy; Beaver, James; Róman Paoli, Elvi; Department of Crops and Agro-Environmental Sciences; Rivera, Aixa
    The tepary bean is a crop that has great potential for expansion in hot and dry regions of Latin America, the Caribbean and Africa, regions that are most vulnerable to the effects of climate change. The tepary bean also has the potential to improve the heat and drought tolerance of common beans (Phaseolus vulgaris L.). A tepary diversity panel (TDP) of 315 tepary accessions from CIAT, USDA and USDA-ARS-TARS and 2 interspecific populations (INB and SK), derived from a Mesoamerican and Andean respectively, common bean recurrent parents, were evaluated for their ability to nodulate with Rhizobium and Bradyrhizobium strains. Strains were evaluated from collections from USDA, CIAT and Zamorano, and from cultures isolated from 3 locations in Puerto Rico. Experiments were conducted under greenhouse conditions, with seed planted in benches filled with sand for the assessment of biological nitrogen fixation. A Randomized Complete Block Design was used with a total of 3 blocks and 4 plants per block in each experiment. Results were analyzed by ANOVA and means were compared using the LSD (P≤0.05) test. Tepary accessions G40142 and G40161 were superior in their ability to nodulate when inoculated with strain USDA 3254. Interspecific lines with transgressive segregation, according to the established nodulation parameters evaluated, were found. Interspecific lines INB 817 and A5-6 had superior nodulation values. Phylogenetic analyzes were conducted based on nodC, atpD and recA genes, using the Neighbour-Joining method. Additional evaluations were conducted by inoculating the tepary diversity panel with the NL3 strain of bean common mosaic necrosis virus (BCMNV) and visually scoring the symptoms. An ELISA test was also used to detect the virus in inoculated plants. The BCMNV screening was conducted under greenhouse conditions during the warm season when high temperatures are prevalent. Tepary bean genotypes G40085, G40099, G40171, and G40178 showed no symptoms in response to inoculation with the strain BCMNV NL3 D, indicating their resistance to the strain evaluated.