López-Soberal, Lorraine

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Detection of anthelmintic resistance in nematodes infecting dairy heifers and evaluation of molecular markers presumably associated with nematode parasite resistance in dairy heifers
(2013) López-Soberal, Lorraine; Ortiz-Colón, Guillermo; College of Agricultural Sciences; Pagán-Morales, Melvin; Jiménez-Cabán, Esbal; González, Olga; Latorre, José R.; Department of Animal Science; Omar Soto, Jay
The presence of anthelmintic resistance was evaluated in 21 dairy commercial farms in Puerto Rico. A total of 407 heifers, with an average age of 9 months, were used to determine efficacy of three anthelmintic treatments: albendazole (ALB), doramectin (DOR) and ivermectin (IVM) in a fecal egg count reduction test (FECRT). Of all anthelmintics used, ALB proved to be the most effective treatment with a 67.47% of efficacy, followed by DOR with a reduction of 25.50%. Fecal egg count reduction (FECR) for IVM was -8.13. Fecal cultures from 10 of the evaluated farms were used to determine the predominant species of gastrointestinal nematodes present in evaluated dairy heifers. Cooperia spp. was the most predominant specie observed (70.88%) followed by Oesophagostomum radiatum (20.00%) and Haemonchus placei (4.63%). FECRT results indicate that anthelmintic resistance is a widespread problem in Puerto Rico.