Arenas-Buitrago, Yolima A.

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    Paecilomyces lilacinus (Thom) Samson y Trichoderma harzianum (Rifai) antagonistas del nematodo de quiste de la soya Heterodera glycines (Ichinohe) presentes en suelos de Puerto Rico
    (2014) Arenas-Buitrago, Yolima A.; Vargas-Ayala, Roberto; College of Agricultural Sciences; Rivera Vargas, Lydia I.; Valencia, Elide; Department of Crops and Agro-Environmental Sciences; Rivera, Rosita
    Soil samples were collected in four areas of Puerto Rico; three forest areas and one agricultural area cultivated with soybeans in order to isolate and identify antagonistic fungi to the soybean cyst nematode, Heterodera glycines (SCN). The fungi were isolated in semi-selective culture media using the serial dilutions of soil method. Fungi with nematode antagonistic characteristics were purified and retained in a culture medium of potato dextrose agar (PDA) with chitin. Four isolates of Paecilomyces lilacinus from soil samples collected in the forest area were identified; also a Trichoderma harzianum was isolated from samples of the area cultivated with soybeans. Subsequently, antagonism tests were conducted in the laboratory and greenhouse on eggs and juvenile larvae of SCN for three planting dates. To determine the degree of parasitism, the alginate films technique was used and fungal growth on cysts, larvae and eggs of the nematode was observed. In addition, greenhouse tests were conducted with soybean plants to examine the level of nematode control and the effect on plants. The results at both laboratory and greenhouse tests, showed antagonistic activity of five fungi on cysts, eggs, and larvae of SCN. Results showed that the morpho-species P. lilacinus had the highest incidence of parasitism on eggs and some effect on the larvae. The TN9P isolated from a forest area showed the best value regarding to results on the best the eggs parasitism. Fungi showed no negative effects on the development of soybean plants; in the case of T. harzianum and stimulation of the root growth was registered. These results show that isolated fungi have potential for use in a management program of SCN.