Soto Morales, Josué

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    Desarrollo de protocolo de multiplicación in vitro de Dioscorea rotundata cv Guinea Negro utilizando biorreactores de inmersión temporal
    (2024-05-10) Soto Morales, Josué; Feliciano Rivera, Merari; College of Agricultural Sciences; Morales Payán, Pablo; Román Paoli, Elvin; Department of Agro-Environmental Sciences; Rúa de la Asunción, Armando
    Yam is one of the most important crops within the group of roots and tubers in Puerto Rico. Due to the lack of disease-free yam seed, local production has not increased in recent years. The objective of this research was to design a protocol for the micropropagation of Dioscorea rotundata cv Guinea Negro using temporary immersion bioreactors, as part of a plan for disease-free seed production. Through the experiments carried out, it was determined that using 1 g/L of Activated Charcoal (AC) during the in vitro establishment of yam resulted in higher multiplication rates than when AC is not used. It was also determined that in the multiplication stage using the temporary immersion bioreactor system (TIS) and 1 g/L of AC results in multiplication rates of 4 buds per plantlet. The plantlets developed in TIS had acclimatization survival rates greater than 95%. Adding 1 mg/L of 6-benzylaminopurine a during multiplication in TIS with AC resulted in a multiplication rate of 4.4 buds per plantlet. It was also determined that plants developed in TIS and then hardened can produce around 8 tubers per plant and half of these can be used directly to produce yam seeds. The results obtained in this research can serve as a basis for carrying out a disease-free seed production plan for farmers in Puerto Rico.