Rosado Díaz, Paula

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    Bacterias antagonistas del nemátodo de quiste de la soya, Heterodera glycines, aisladas de un suelo en Puerto Rico
    (2014) Rosado Díaz, Paula; Vargas Ayala, Roberto; College of Agricultural Sciences; Nadathur, Govind S.; Valencia, Elide; Department of Crops and Agro-Environmental Sciences; Kolterman, Duane A.
    Soy bean cyst nematode ( Heterodera glycines) was reported in Puerto Rico in 1999. This represents a threat to the cultivation of soybean Glycine max. Although the parasite is in one location, it is necessary to know if there are natural enemies that could exert some control of the nematode on the island. The purpose of this research was to find biocontrol agents in Puerto Rican soil with the ability to control root nematode infection. For the selection of potential biocontrol agents the King B test was performed in order to find strains of Pseudomonas which has been catalogued with antagonistic properties. After reviewing certain physiological characteristics of soybean cyst nematode (SCN), between these layers comprising chitin and vitellin body, two generous of bacteria with degradative properties of these compounds in vitro tests were selected. These properties were confirmed by growing the bacteria in chitin and casein media respectively. These were identified, using the sequencing method of the 16S ribosomal DNA region, such as Pseudomonas sp. NJ-22. and Serratia marcescens, both used in previous studies for the control of Meloidogyne spp. in bean plants. In this research, the bacteria Pseudomonas sp. NJ-22 met the requirements to control nematode infection at the root of soybean cultivar Lee 74 without harming the plant. However, it did not present severe infection in direct contact with larvae and eggs. Serratia marcescens was not effective to prevent infection by J2 larvae of roots of soybean plants, however, effective growth and colonization on eggs and larvae of SCN were observed. In conclusion, both bacteria act differently in controlling the nematode, although it would be necessary to elaborate these findings for use at field level.