Dillon-Fuentes, Lisa M.

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    Effects of culture media on the in vitro maturation rate of bovine oocytes
    (2015) Dillon-Fuentes, Lisa M.; Fernández-Van Cleve, John; College of Agricultural Sciences; Rodríguez Sallaberry, Carlos; Jiménez Cabán, Esbal; Department of Animal Science; Latorre, José R.
    Puerto Rico is located in a tropical zone characterized by a relatively constant high heat and humidity. As a result, cattle are under moderate to severe thermal stress on a year-round basis, depending on the time of the year. Thus, the problem of thermal stress can be considered chronic and one of the net results is a decrease in reproductive efficiency. The objective of this experiment was to study the feasibility and thermo- tolerance of in vitro matured oocytes collected in different seasons. For this, 87 dairy-breed ovaries (Holstein) and 120 beef-breed ovaries (Senepol, Zebu and cross breeds) were obtained in abattoirs Ganaderos Santiago and Hermanos Alvarado during four seasons [December-February (S1; 20°C - 29°C), March and April (S2; 20°C - 30°C), June to August (S3; 22°C - 32°C) and September to November (S4; 22°C - 31°C)]. A total of 804 oocytes were collected from 2-8 mm follicles. These were placed into three maturation mediums with different concentrations of Pluset (FSH y LH) and Estradiol (E2) (A: Pluset 5µg/µl and Estradiol 25µM; B: Pluset 25µg/µl and Estradiol 25µM; C: Pluset 5µg/µl) and matured in vitro for 22 hrs. in an incubator under a 5% CO2 environment at 37.5°C. The oocytes in medium A (lower concentration of LH, FSH and presence of E2) had a 73% maturation than those in medium B and C [59% average maturity; P <0.05]. Oocytes from beef type cows had better maturation rates when compared with those from Holstein cows during S4 [80% (beef) vs. 60% (dairy); P <0.05]. Beef cattle also demonstrated better maturation rates in S4 [80%] than dairy cattle in S3 [50%]. In turn, oocyte maturation from beef type cattle showed an increase in maturation during S2 [55%] to S3 [68%] and again in S4 [80%] while dairy cattle drastically reduced 21% in maturation from S2 [71%] to S3 [50%]; (P <0.05). These results suggest that beef cattle produce oocytes better adapted to tropical conditions and have better maturation rates in culture mediums with low gonadotropins concentrations and high concentrations of E2.