Miranda Vélez, Magdiel A.

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    Disease assessment, inferences on virus movement, and seedling development in tropical pumpkin (Cucurbita moschata) infected with papaya ringspot virus and zucchini yellow mosaic virus
    (2018-05) Miranda Vélez, Magdiel A.; Verle Rodrígues, José C.; College of Arts and Sciences - Sciences; Wessel Beaver, Linda; Ferwerda, Feiko H.; Vargas Ayala, Roberto; Department of Crops and Agro-Environmental Sciences; Ríos López, Isabel
    Papaya ringspot virus (PRSV) and Zucchini yellow mosaic virus (ZYMV) frequently infect tropical pumpkin (Cucurbita moschata) in Puerto Rico. Breeding programs depend on the development of efficient and reliable methods of assessing resistance. The goal of this research was to determine the best method to differentiate the level of resistance among genotypes of tropical pumpkin for both PRSV and ZYMV, determine if there are differences in seedling development of C. moschata infected with PRSV, ZYMV, or both PRSV+ZYMV, and to evaluate whether ‘Menina’ can serve as a source of resistance. The cotyledons of seven genotypes were inoculated with PRSV, ZYMV or PRSV+ZYMV. Analysis of the viral serological titer in the first five leaves was performed using ELISA tests. An ELISA reading <0.400 (A405nm) was considered to be negative for presence of virus. Symptoms and fresh and dry weight were taken. Small leaves, intervenal chlorosis, leaf deformation, curled leaves, and mosaic were some of the symptoms observed in susceptible genotypes inoculated with PRSV and ZYMV. Smaller leaves and chlorosis were observed in ‘Menina’ inoculated with PRSV and ZYMV and no symptoms were observed in ‘Nigerian Local’. For PRSV, sampling of the fourth leaf was required to differentiate between resistant versus susceptible genotypes. For ZYMV, leaves 2, 3, and 4 can be used to differentiate resistant from susceptible genotypes. No differences were found in ELISA readings from plants with single versus double inoculation. No differences in fresh and dry weight were found between uninoculated versus inoculated plants. Negative ELISA readings were obtained in the inoculation of susceptible genotypes with sap of inoculated resistant genotypes. ‘Menina’ was observed to be a useful source of resistance to ZYMV and PRSV. The results of this study can be used when developing a breeding program for resistance to these two viruses.