Atienza-Ramos, Patricia B.

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    Expression of pregnancy-specific beta-1-glycoprotein, aromatase and nitric oxide synthase in bovine sperm
    (2014) Atienza-Ramos, Patricia B.; Jiménez-Cabán, Esbal; College of Agricultural Sciences; Pagán-Morales, Melvin; González-Vélez, Olga; Latorre-Acevedo, José R.; Department of Animal Science; Flores Mangual, Mario L.
    In the search for alternative indicators of fertility in bulls, this investigation evaluated the effects of breed, type of cattle and age, on the relative expression of the genes Aromatase, Pregnancy- Specific-Beta-1-Glycoprotein (PSG1), and Nitric Oxide Synthase (NOS) in bovine semen. Sperm from Jersey, Holstein, Brahman, Brangus, Charolais, Charbray, Senepol, and Simmental bulls was collected and extracted mRNA was analyzed for expression of the genes by using Reverse transcriptase and Real Time Polymerase Chain Reaction (RT-PCR). The relative expression of aromatase was lower (P<0.07) in sperm from Jersey bulls (0.89) than in bulls of all other breeds (average 1.01). The relative expression of PGS1 was greater (P>0.02) in sperm from dairy (1.00) than from beef bulls (0.96). Age did not affect relative expression of the genes investigated. Additionally, in data from thirty-one bulls, expression of NOS was positively correlated with scrotal circumference (r=0.31; P<0.08). To our knowledge, these are the first results showing that the genes aromatase, PSG1 and NOS can be detected in bull sperm and may have implications as molecular markers for bull fertility.