Faría Medina, Odáliz
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Publication Aislamiento de cepas de Bacillus spp. provenientes de suelos boscosos de Puerto Rico y su potencial como antagonista del nemátodo nodulador Meloidogyne spp.(2013) Faría Medina, Odáliz; Vargas Ayala, Roberto; College of Agricultural Sciences; Alameda Lozada, Myrna Z.; Rivera Vargas, Lydia; Department of Crops and Agro-Environmental Sciences; Fernández Van Cleve, John A.The objective of this research was to identify native bacteria strains with antagonistic properties against juvenile and eggs of tomato root-knot nematode (Meloidogyne spp.). Two sampling sites located in the rainforest in the center in the island were selected. One of them was Toro Negro at Jayuya and the other one was Cerro Guilarte at Adjuntas. Criteria related to the bacterial growth on media were used to select two colonies from each sampling site. Biochemical tests such as gram staining, green malachite, catalase, oxidase, and KOH was used to characterize bacterial isolates Antagonism potential of those selected bacterial was evaluated in vitro and greenhouse assay. Results showed that treatments with bacterial strains GU-2.1 and GU-4.5 decreased Meloidogyne spp. eggs hatch in vitro compare to the control (=0.05). In contrast, greenhouse assays showed that these tomato plants inoculated with Meloidogyne spp. and treated with bacterial suspension of GU-2 strain and GU-4.5 did not showed differences (=0.05) of the famele inside the root. But difference (=0.05) were observed in the number of plant parasitic nematodes counted in soil after GU-2.1 treatment application. This treatment showed a reduction of plant parasitic nematode population in the soil. According to the Biolog data base, bacterial strains used in our assay were identified as Bacillus thuringiensis.