Feliciano Caraballo, Idainés

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    Synthesis, characterization, binding interactions with biomolecules and cytotoxic studies of molybdebocene complexes
    (2009) Feliciano Caraballo, Idainés; Meléndez, Enrique; College of Arts and Sciences - Sciences; Cádiz, Mayra E.; Román Velázquez, Félix R.; Vega Olivencia, Carmen A.; Department of Chemistry; Velásquez, Carlos
    Bis-(cyclopentadienyl) molybdenum dichloride or molybdenocene dihalides, like other metallocenes dihalides, have shown antitumor properties in different human tumors. In contrast to titanocene dichloride, molybdenocene dichloride has a higher water solubility and high stability under physiological pH conditions. The present work was aimed to synthesize and characterize molybdenocene complexes, to study their interactions with biologically important molecules such as calf-thymus DNA or human serum albumin (HSA) and to evaluate their cytotoxic activity. Two complexes were synthesized: Cp2Mo(malonate) and [Cp2Mo(maltolato)]Cl. Both complexes were characterized spectroscopically by NMR, IR and Mass Spectrometry. Also, an electrochemical characterization was pursued using cyclic voltammetry. The interactions between the molybdenocene complexes with calf-thymus DNA and with HSA, in buffer conditions, were studied by cyclic voltammetry. Binding studies by cyclic voltammetry experiments showed a possible interaction of the molybdocene dichloride with albumin and very weak, almost negligible interaction with DNA. The malonate and maltolato complexes did not show any interaction with DNA or HSA. In order to assess, in more details, the interaction of molybdenocene complexes with DNA or HSA we performed titrations using UV-Vis spectrometry, but no conclusive evidence of interaction can be extrapolated from these titrations. The cytotoxic activities of the molybdenocene dichloride and of the synthesized complexes were investigated on the HT-29 colon cancer cell line and on the MCF-7 human breast cancer cell line. Also the cytotoxic activities of the vanadocene dichloride and [Cp2V(maltolato)]Cl complex on HT-29 were studied.