De La Mota Peynado, Alina M.

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Infectious capability of the plus (+) and minus (-) strands of parvovirus LUIII
(2004) De La Mota Peynado, Alina M.; Diffoot Carlo, Nanette; College of Arts and Sciences - Sciences; Velázquez, Carlos Ríos; Buxeda, Rosa; Department of Biology; Riquelme, Ernesto
The replication of autonomous parvovirus LuIII and its encapsidation pattern have not been fully explained. Two genomic clones of LuIII, pBLu(+) and pBLu(-), were constructed. These clones were used to produce the (+) or (-) ss viral DNA, to assess the capability of infection of the individual strands. Three different techniques were utilized to obtain the single-stranded LuIII DNA. Only the treatment with Exonuclease III yielded both strands from the pBLu(+) construct. Each polarity of the single-stranded LuIII DNA was independently transfected into cancerous HeLa cells by electroporation. The results obtained suggest that both the ss(+) and ss(-) LuIII DNA were capable of establishing an infection in these cells. The (-) strand produced 100% cell lysis two days prior to the (+) strand, appearing to be more effective in the establishment of viral infection.