Publication:
Distribución vertical de Glomerella cingulata (Colletotrichum gloeosporioides) sobre una siembra comercial de gandul (Cajanus cajan L.)

dc.contributor.advisor Maldonado-Ramírez, Sandra L.
dc.contributor.author Gaztambide-Alameda, Idarnis
dc.contributor.college College of Arts and Sciences - Sciences en_US
dc.contributor.committee Rivera Vargas, Lydia I.
dc.contributor.committee Montalvo Rodríguez, Rafael R.
dc.contributor.department Department of Biology en_US
dc.contributor.representative Bosques Vega, Angel
dc.date.accessioned 2018-10-05T17:55:50Z
dc.date.available 2018-10-05T17:55:50Z
dc.date.issued 2005
dc.description.abstract Pigeon pea [Cajanus cajan (L.) Millsp.], a crop of great importance in tropical countries, is affected by pod anthracnose, caused by Colletotrichum spp. The objectives of this investigation were to study the vertical and temporal distribution and incidence of Colletotrichum spp. on a pigeon pea field. A sampling tower was designed to accommodate Petri plates with oatmeal agar and exposed them for ten minutes. Alternaria, Aspergillus, Cladosporium Curvularia, Drechslera, Fusarium and Penicillium prevailed in all samplings. All Colletotrichum gloeosporioides colonies were recuperated during the morning hours, at 94, 180, 190 cm from the ground, with a frequency of 0.009. All C. gloeosporioides isolates examinated were pathogenic to pods and presented by PCR amplification, a band of 600 pb molecular weight, approximately. RFLP analysis presented three band pattern for all isolates. Sequencing analysis of DNA showed that isolates belong to G.cingulata. en_US
dc.description.abstract El gandul [Cajanus cajan (L.) Millsp.] es un cultivo de gran importancia en países tropicales, que es afectado por la antracnosis de la vaina causada por Colletotrichum spp. Esta investigación tuvo como propósito estudiar la distribución vertical y temporal e incidencia de Colletotrichum spp. sobre una siembra comercial. Se colocaron platos Petri con agar de avena en una torre y se expusieron durante diez minutos. Colletotrichum. Alternaria, Aspergillus, Cladosporium Curvularia, Drechslera, Fusarium y Penicillium, prevalecieron en todos los muestreos. Las colonias de Colletotrichum gloeosporioides fueron recuperadas en la mañana, a 94, 180, 190 cm del suelo, con una frecuencia de 0.009. Todos los aislados de C. gloeosporioides estudiados presentaron, mediante PCR, una banda de un peso molecular de aproximadamente 600 pb. El análisis de RFLP mostró un patrón de tres bandas para todos los aislados. Un análisis de secuenciación del ADN determinó que los aislados pertenecen a G.cingulata. en_US
dc.description.graduationYear 2005 en_US
dc.identifier.uri https://hdl.handle.net/20.500.11801/1002
dc.language.iso es en_US
dc.rights.holder (c) 2005 Idarnis Gaztambide Alameda en_US
dc.rights.license All rights reserved en_US
dc.subject Gandul en_US
dc.subject Cajanus cajan (L.) Millsp. en_US
dc.subject Colletotrichum spp. en_US
dc.subject G.cingulata en_US
dc.subject.lcsh Pigeon pea--Diseases and pests. en_US
dc.subject.lcsh Glomerella cingulata. en_US
dc.subject.lcsh Anthracnose. en_US
dc.title Distribución vertical de Glomerella cingulata (Colletotrichum gloeosporioides) sobre una siembra comercial de gandul (Cajanus cajan L.) en_US
dc.type Thesis en_US
dspace.entity.type Publication
thesis.degree.discipline Biology en_US
thesis.degree.level M.S. en_US
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