Publication:
Distribución vertical de Glomerella cingulata (Colletotrichum gloeosporioides) sobre una siembra comercial de gandul (Cajanus cajan L.)
Distribución vertical de Glomerella cingulata (Colletotrichum gloeosporioides) sobre una siembra comercial de gandul (Cajanus cajan L.)
dc.contributor.advisor | Maldonado-Ramírez, Sandra L. | |
dc.contributor.author | Gaztambide-Alameda, Idarnis | |
dc.contributor.college | College of Arts and Sciences - Sciences | en_US |
dc.contributor.committee | Rivera Vargas, Lydia I. | |
dc.contributor.committee | Montalvo Rodríguez, Rafael R. | |
dc.contributor.department | Department of Biology | en_US |
dc.contributor.representative | Bosques Vega, Angel | |
dc.date.accessioned | 2018-10-05T17:55:50Z | |
dc.date.available | 2018-10-05T17:55:50Z | |
dc.date.issued | 2005 | |
dc.description.abstract | Pigeon pea [Cajanus cajan (L.) Millsp.], a crop of great importance in tropical countries, is affected by pod anthracnose, caused by Colletotrichum spp. The objectives of this investigation were to study the vertical and temporal distribution and incidence of Colletotrichum spp. on a pigeon pea field. A sampling tower was designed to accommodate Petri plates with oatmeal agar and exposed them for ten minutes. Alternaria, Aspergillus, Cladosporium Curvularia, Drechslera, Fusarium and Penicillium prevailed in all samplings. All Colletotrichum gloeosporioides colonies were recuperated during the morning hours, at 94, 180, 190 cm from the ground, with a frequency of 0.009. All C. gloeosporioides isolates examinated were pathogenic to pods and presented by PCR amplification, a band of 600 pb molecular weight, approximately. RFLP analysis presented three band pattern for all isolates. Sequencing analysis of DNA showed that isolates belong to G.cingulata. | en_US |
dc.description.abstract | El gandul [Cajanus cajan (L.) Millsp.] es un cultivo de gran importancia en países tropicales, que es afectado por la antracnosis de la vaina causada por Colletotrichum spp. Esta investigación tuvo como propósito estudiar la distribución vertical y temporal e incidencia de Colletotrichum spp. sobre una siembra comercial. Se colocaron platos Petri con agar de avena en una torre y se expusieron durante diez minutos. Colletotrichum. Alternaria, Aspergillus, Cladosporium Curvularia, Drechslera, Fusarium y Penicillium, prevalecieron en todos los muestreos. Las colonias de Colletotrichum gloeosporioides fueron recuperadas en la mañana, a 94, 180, 190 cm del suelo, con una frecuencia de 0.009. Todos los aislados de C. gloeosporioides estudiados presentaron, mediante PCR, una banda de un peso molecular de aproximadamente 600 pb. El análisis de RFLP mostró un patrón de tres bandas para todos los aislados. Un análisis de secuenciación del ADN determinó que los aislados pertenecen a G.cingulata. | en_US |
dc.description.graduationYear | 2005 | en_US |
dc.identifier.uri | https://hdl.handle.net/20.500.11801/1002 | |
dc.language.iso | es | en_US |
dc.rights.holder | (c) 2005 Idarnis Gaztambide Alameda | en_US |
dc.rights.license | All rights reserved | en_US |
dc.subject | Gandul | en_US |
dc.subject | Cajanus cajan (L.) Millsp. | en_US |
dc.subject | Colletotrichum spp. | en_US |
dc.subject | G.cingulata | en_US |
dc.subject.lcsh | Pigeon pea--Diseases and pests. | en_US |
dc.subject.lcsh | Glomerella cingulata. | en_US |
dc.subject.lcsh | Anthracnose. | en_US |
dc.title | Distribución vertical de Glomerella cingulata (Colletotrichum gloeosporioides) sobre una siembra comercial de gandul (Cajanus cajan L.) | en_US |
dc.type | Thesis | en_US |
dspace.entity.type | Publication | |
thesis.degree.discipline | Biology | en_US |
thesis.degree.level | M.S. | en_US |