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Abstract
Biosensors are becoming increasingly important as practical tools in pathogen detection and
molecular diagnostics. Electrochemical biosensors are of particular interest due to several
advantages including low-cost, easy operation, and small size. During the development of
efficient biosensors, a crucial part is the choice of material onto which a biomolecule has to be
immobilized while providing good electron transfer rate at the electrode as a result of a
biochemical reaction.
Present work deals with the development of novel nanostructured metal oxides and conducting
polymer nanocomposites as promising material matrix for biomolecule immobilization, because
of their high surface to volume ratio and efficient electron transport properties.
We have synthesized ferrocene-polypyrrole based nanocomposites with excellent mechanical
and electronic properties. The optimized nanocomposite films have been used for glucose
biosensor. Results indicate the good performance of the electropolymerized copolymer of pyrrole
and ferrocenecarboxylate modified pyrrole P(Py-FcPy) on indium-tin-oxide (ITO) coated glass
for glucose biosensor.
On the other hand an inorganic material structure, ZnO nanorods (ZnONR), has been grown on
indium tin oxide (ITO) films on glass. This could easily be upgraded with existing MEMS
technology to fabricate miniaturized biosensors. Urease (Urs) enzyme was physically
immobilized on nanorods exploiting high isoelectric point of ZnO to fabricate Urs/ZnONR/ITO
bioelectrode. Urs/ZnONR/ITO bioelectrode revealed a very high sensitivity of 10 µAdL/mg
(1.66 µA/mM) in a urea concentration range of 10-25 mg/dl with enhanced linearity.
Description
Date
2011
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Keywords
biosensors