Molecular and morphological characterization of Rhodobacter sphaeroides 7001 bacteriophages øR3-PR6 and øR3-PR7

dc.contributor.advisor Ríos-Velázquez, Carlos Román-Rodríguez, Franklin College of Arts and Sciences - Sciences en_US
dc.contributor.committee Martínez-Cruzado, Juan C.
dc.contributor.committee Rodríguez-Minguela, Carlos M.
dc.contributor.department Department of Biology en_US
dc.contributor.representative Orellana-Feliciano, Lynette E. 2020-08-19T14:45:12Z 2020-08-19T14:45:12Z 2020-05-26
dc.description.abstract Bacteriophages are ubiquitous viral particles found in most environments, such as oceans, rivers, and soil. This wide distribution makes phages one of the most abundant viral types on the planet. Bacteria are outnumbered by phages by a factor of 10 to 1, totaling a population close to c capable of conducting an estimated a 1023 infections per seconds globally. Bacteriophages have been widely studied, especially in gram-positive and delta-proteobacteria. However, information regarding phages belonging to the Rhodospirillaceae family is scarce. The øR3-PR1-5 are bacteriophages capable of infecting Rhodobacter sphaeroides 7001 and was originally isolated by Rojas et al., 2009 in different freshwater bodies in southwest Puerto Rico. Since the isolations, two more bacteriophages specific for a restriction system mutant of Rhodobacter sphaeroides have been isolated in aquatic environments int northern part of the Island and have been named øR3-PR6 and øR3-PR7. This study describes molecularly and morphologically both phages and compare them with the other five isolated Rhodophages (bacteriophages that infect Rhodobacter spp.) in Puerto Rico. Transmission electron microscopy revealed that both phages differ in morphology, capsid and tail size. Furthermore, morphological analysis suggests both phages belong to the Caudovirales order whereas øR3-PR6-7 seems to be affiliated to the Siphoviridae family. Restriction enzyme digestion analyses using ClaI and HindIII shows that these phages present distinctive banding pattern. Estimated genome size for each phage is about 53.236 KbP in øR3-PR6 and 51.865 KbP in øR3-PR7. Total genome DNA sequencing and subsequent Rapid Annotation using Subsystem Technology (RAST) analysis demonstrated that Rhodophages share various putative proteins amongst themselves and with Rhizobium phage vB_Rgls_P106B. This study expands on the limited knowledge available for Rhodophages isolated outside of the United States and provides information applicable to the advancement of phage mediated genetic transfer studies across the Rhodospirillaceae as well as a deeper understanding of the Siphoviridae family. en_US
dc.description.graduationSemester Spring en_US
dc.description.graduationYear 2020 en_US
dc.description.sponsorship NIH Bridges to the Doctorate Program (NIH Grant # 4R25GM058389-16) en_US
dc.language.iso en en_US
dc.rights.holder (c) 2020 Franklin Román-Rodríguez en_US
dc.subject bacteriophage en_US
dc.subject phage morphology en_US
dc.subject molecular analysis en_US
dc.subject rhodobacter sphaeroides en_US
dc.subject rhizobium en_US
dc.title Molecular and morphological characterization of Rhodobacter sphaeroides 7001 bacteriophages øR3-PR6 and øR3-PR7 en_US
dc.type Thesis en_US
dspace.entity.type Publication Biology en_US M.S. en_US
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