Publication:
Effects of thermal oxidation of gamma titanium aluminide on human osteoblast adhesion
Effects of thermal oxidation of gamma titanium aluminide on human osteoblast adhesion
Authors
Bello-Melo, Samir A.
Embargoed Until
Advisor
Diffoot-Carlo, Nanette
College
College of Arts and Sciences - Sciences
Department
Department of Biology
Degree Level
M.S.
Publisher
Date
2009
Abstract
The corrosion resistance of gamma titanium aluminide (gamma-TiAl), a potential
implant material, is increased in vitro by thermal oxidation. Although the biocompatibility of
autoclaved gamma-TiAl was previously demonstrated, the osteoblast response to thermally
oxidized gamma-TiAl has not been addressed to date. Thus, the adhesion of human osteoblasts to
oxidized gamma-TiAl surfaces was examined in vitro. Cell adhesion on thermally oxidized Ti6Al-4V
was evaluated for comparison. hFOB 1.19 cells were seeded on autoclaved (GTi, TiV),
thermally oxidized at 500ºC (GTi5, TiV5), and at 800ºC (GTi8, TiV8) gamma-TiAl and Ti-6Al4V
disks, respectively. A hexosaminidase assay, Scanning Electron Microscopy (SEM) and
immunofluorescent labeling of β1 integrin and vinculin were used to evaluate cell adhesion on
the different surfaces. Hexosaminidase assay showed no differences in cell attachment on all
surfaces 1 day postseeding, but fewer cells were attached on TiV8 compared to those attached on
the other surfaces, 7 and 14 days postseeding. Expression of β1 integrin and vinculin in cells
cultured for 7 days on all the surfaces, except on TiV8, was demonstrated by immunofluorescent
labeling. SEM images showed that cells adhered on all but TiV8 surfaces, 14 days postseeding.
Taken together, the results indicate that hFOB 1.19 cells were able to attach and proliferate on
autoclaved and both thermally oxidized gamma-TiAl surfaces in a similar manner, suggesting its
biocompatibility. On the contrary, TiV8 surfaces exhibit cytotoxic effects on hFOB 1.19 cells in
a time dependent manner.
Keywords
Corrosion resistance of gamma titanium aluminide (gamma-TiAl),
Thermal oxidation
Thermal oxidation
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Cite
Bello-Melo, S. A. (2009). Effects of thermal oxidation of gamma titanium aluminide on human osteoblast adhesion [Thesis]. Retrieved from https://hdl.handle.net/20.500.11801/240