Effect of Beta-lactoglobulin in oxidatively stressed yeast Saccharomyces cerevisiae cells

dc.contributor.advisor Latorre-Esteves, Magda Rivera-Baigés, José C. College of Agricultural Sciences en_US
dc.contributor.committee Latorre, José R.
dc.contributor.committee Ponce de León, Leyda
dc.contributor.department Department of Animal Science en_US
dc.contributor.representative Rivera, Rosita 2019-07-01T19:32:45Z 2019-07-01T19:32:45Z 2019-04-12
dc.description.abstract Beta-lactoglobulin (β-Lg) is the most abundant protein in the serum of bovine milk. However, it should be mentioned that this protein is absent in human milk. According to previous studies, the peptides contained in β-Lg are precursors of glutathione. Glutathione is a powerful antioxidant produced by human cells to maintain oxidation- reduction balance. Therefore, we theorize that β-Lg may have antioxidant effects. There are no studies in the literature that test this idea. The objectives of this study are to establish a method to induce oxidative stress in yeast Saccharomyces cerevisiae and to evaluate if β-Lg exerts a protective effect on these oxidatively challenged cells. First, we needed to determine if β-Lg had an effect on cellular viability. None of the concentrations tested had an effect on the viability of cells (P=0.1674). Different ways of inducing oxidative stress in yeast were analyzed, among them: exposure to C-type ultraviolet radiation, dihydrogen peroxide, menadione and menadione sodium bisulfite (MSB). The compound that produced the most reliable results was MSB. 40% of cell death was achieved using 0.5 mM MSB. MSB-treated cells were co-incubated with 6.25 mg / mL of β-Lg. The results show that the yeast treated with β-Lg had a 10.94% higher surviving rate than the cells with no β-Lg (P <0.0001). These results suggest that the β-Lg protein exerts a protective effect on oxidatively stressed cells. en_US
dc.description.graduationSemester Spring en_US
dc.description.graduationYear 2019 en_US
dc.language.iso en en_US
dc.rights Attribution-NonCommercial-NoDerivs 3.0 United States *
dc.rights.holder (c) 2019 José C. Rivera-Baigés en_US
dc.rights.uri *
dc.subject Beta-lactoglobulin en_US
dc.subject oxidative stress en_US
dc.subject antioxidant en_US
dc.subject.lcsh Lactoglobulines en_US
dc.subject.lcsh Milk proteins en_US
dc.subject.lcsh Oxidative stress en_US
dc.subject.lcsh Saccharomyces cerevisiae en_US
dc.subject.lcsh Glutathione en_US
dc.title Effect of Beta-lactoglobulin in oxidatively stressed yeast Saccharomyces cerevisiae cells en_US
dc.type Thesis en_US
dspace.entity.type Publication Animal Industry en_US M.S. en_US
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