Development and characterization of a multi-cellular in vitro model for the study of nanoparticle-cell Interface

dc.contributor.advisor Resto-Irizarry, Pedro J. Agostini-Infanzon, Reinaldo J. College of Engineering en_US
dc.contributor.committee Domenech, Maribella
dc.contributor.committee Díaz, Rubén
dc.contributor.department Department of Mechanical Engineering en_US
dc.contributor.representative Perales, Oscar 2019-03-29T12:58:00Z 2019-03-29T12:58:00Z 2018-12-12
dc.description.abstract Triple Negative Breast Cancer (TNBC) is distinguished by various characteristics which are associated to its poor prognosis. This type of cancer is aggressive in nature and has no targeted therapies. The tumor microenvironment has shown to modulate tumor cell behavior and response to clinical therapies. Current in vitro technologies allow us to observe multicellular interactions for extended periods; however, these present a variety of limitations including the need for cell sorting, requirement of large amounts of cells, and lack of tunable discrete adjacent compartments. Other technologies such as microfluidic platforms lack a user-friendly interface and require specialized instrumentation and training, making it difficult to be routinely used in a cell biology laboratory. To overcome these problems, an open multi-microwell triculture device was manufactured to probe tumor-stromal interactions. The devices were printed out of polystyrene sheets using a razor-blade plotter and manually layered into a 3-dimensional structure. We have evaluated the effect of normal and cancerous stromal cell clusters (fibroblasts and macrophages) in modulating tumor behavior in MDA-MB-231 cells. Tumor cell proliferation was evaluated at 72hrs cultured with stromal clusters under exposure to stress conditions. Results for normal stromal cell clusters show no significant difference in proliferation at 37°C and 41°C. On the other hand, cancer associated cells show an increased proliferation rate of 11.7% for 37°C than for 41°C. Normal stroma shows potential damping on the effects of the high temperature stress on the cells, as no significant difference was observed. Thus, normal stroma improves recovery from heat damage as compared to cancer-stroma. Multi-adjacent microwell stickers are a fast prototyping culture platform and provide flexibility for testing diverse biomaterials with varying conditions for each cell type in multi-culture cell signaling studies. en_US
dc.description.graduationSemester Fall en_US
dc.description.graduationYear 2018 en_US
dc.description.sponsorship Investigation subsidized by NSF CREST Grant No. HRD 1345156. en_US
dc.language.iso en en_US
dc.rights.holder (c) 2018 Reinaldo Agostini Infanzón en_US
dc.rights.license All rights reserved en_US
dc.subject Multi-cellular model en_US
dc.subject Magnetic fluid hyperthermia en_US
dc.subject Macrophages en_US
dc.subject Triple negative breast cancer en_US
dc.subject Razor cutter en_US
dc.subject.lcsh Nanoparticles en_US
dc.subject.lcsh Breast -- Cancer en_US
dc.subject.lcsh Biomedical materials en_US
dc.subject.lcsh Cancer cells en_US
dc.title Development and characterization of a multi-cellular in vitro model for the study of nanoparticle-cell Interface en_US
dc.type Thesis en_US
dspace.entity.type Publication Mechanical Engineering en_US M.S. en_US
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