Orientation of collagen fibers by mechanical transduction on osteoblastic cells

dc.contributor.advisor Sundaram, Paul A. Boada-López, Jairo A. College of Engineering en_US
dc.contributor.committee Díaz, Rubén
dc.contributor.committee Diffoot, Nanette
dc.contributor.department Department of Mechanical Engineering en_US
dc.contributor.representative Romañach, Rodolfo J. 2018-09-25T18:41:08Z 2018-09-25T18:41:08Z 2011
dc.description.abstract MC3T3-E1 Osteoblastic cells were mechanically stimulated using a static load of 3, 6 and 9 lb during 2, 4, 6 and 8 days of incubation. The Collagen Type I expression rate was analyzed using the Near Infrared Microscope with Chemical Imaging (NIR-CI)and the alignment of the collagen fibers was observed with Confocal and Scanning Electron Microscope images. Results show that cells increase their collagen production with 3 and 6 lb with a 32.4 and 109 % more than the control sample at 8 days, and also a preferential alignment was achieved near to the direction of the load applied. The 9 lb load had a different effect over the collagen expression, which was lower when compared with the control sample and a preferential alignment was not obtained. en_US
dc.description.abstract Células osteoblasticas MC3T3-E1 fueron mecánicamente estimuladas a través de una carga estática de 3, 6 y 9 libras durante 2, 4, 6 y 8 días de incubación. Para analizar la influencia del estimulo en la producción de colágeno se utilizo el microscopio de infrarrojo cercano con imagen química (NIR-CI) y para determinar la orientación de las fibras se empleo el microscopio confocal invertido y el microscopio de emisión de electrones (SEM). Hubo un aumento considerable en la cantidad de colágeno con las cargas de 3 y 6 libras de 32.4 y 109 % más que la muestra de control al término del periodo de 8 días, y se obtuvo una alineación preferencial de fibras cercano a la dirección de la carga, sin embargo con la carga de 9 libras no se obtuvo ninguna alineación preferencial y la expresión de colágeno fue menor comparada con la muestra de control. en_US
dc.description.graduationSemester Summer en_US
dc.description.graduationYear 2011 en_US
dc.description.sponsorship National Science Foundation in the form of MRI grant award 0821113, which permitted the purchase of the NIR-CI system. en_US
dc.language.iso en en_US
dc.rights.holder (c) 2011 Jairo Andrés Boada López en_US
dc.rights.license All rights reserved en_US
dc.subject osteoblastic cells en_US
dc.subject.lcsh Biomechanics. en_US
dc.subject.lcsh Osteoblasts--Mechanical properties. en_US
dc.subject.lcsh Transduction. en_US
dc.subject.lcsh Collagen. en_US
dc.title Orientation of collagen fibers by mechanical transduction on osteoblastic cells en_US
dc.type Thesis en_US
dspace.entity.type Publication Mechanical Engineering en_US M.S. en_US
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