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dc.contributor.advisorRodríguez-Carías, Abner A.
dc.contributor.authorTous-Rivera, Karla M.
dc.date.accessioned2018-11-17T22:21:29Z
dc.date.available2018-11-17T22:21:29Z
dc.date.issued2007
dc.identifier.urihttps://hdl.handle.net/20.500.11801/1386
dc.description.abstractTwo experiments were conducted to determine the effect of application of exogenous fibrolytic enzymes on chemical composition, voluntary feed intake (VFI), nutrient digestibility, apparent and true in vitro dry matter (DM) degradability of guineagrass hay (GH) (Panicum Maximum Jacq.). The enzymatic products used were Promote NET (Agribrand, Canadá) and Biocellulase A-20 (Loders Croklaan, Channahon, IL, USA), derived from Trichoderma longibratum and Aspergillus reesei, and containing mainly cellulase and xylanase. In the first experiment, nine (9) mature Blackbelly x criollo cross rams of 26.8 Kg mean body weight (BW) were used in a 3x3 Latin Square design, with 18 d periods. Treatments consisted of untreated hay (control), hay treated with PromoteNET and hay treated with BiocellulaseA-20, applied by spraying 24 h prior to feeding. Daily hay offerings were at 4% of BW on a dry matter (DM) basis. Enzyme treatment increased contents of DM and crude protein (CP) in the GH relative to the untreated hay. A tendency to reduce neutral detergent fiber (NDF), acid detergent fiber (ADF) and hemicellulose concentrations through the enzymatic treatments was also observed, but was not significant (P< 0.05). Voluntary intake (VI) of DM of hays treated with BiocellulaseA-20 and PromoteNET (1139 and 938 g/d, respectively) exceeded that of the control (921 g/d). VI of CP, NDF and ADF were higher for hay treated with BiocellulaseA-20, than for untreated GH or hay treated with PromoteNET. Rams fed fortified hay had higher (P< 0.05) DM intake as percentage of BW when compared to rams fed untreated GH (4.06 BiocellulaseA-20, 3.15 PromoteNET and 2.86% control). Daily DM intake as a percentage of BW was higher (P< 0.05) for rams fed GH treated with both enzymatic products than for the control (4.06%, BiocellulaseA-20; 3.15%, PromoteNET vs. 2.86%). Apparent DM digestibility increased (P<0.05) between enzymatic treatments, while NDF digestibility was higher (P< 0.05) for GH treated with BiocellulaseA-20. In the second experiment, apparent in vitro dry matter degradability (IVDMDA) and true in vitro dry matter degradability (IVDMDT) were found to be higher (P< 0.05) for GH treated with both enzymes relative to the control (IVDMDA by 2.75 percentage units for BiocellulaseA20, 2.12 for PromoteNET; IVDMDT by 2.42 and 1.73 units, respectively). These results indicate that application of fibrolytic enzymatic complexes to low-quality tropical grass hay can improve the nutritional quality substantially.
dc.description.abstractSe realizaron dos experimentos para determinar el efecto de la aplicación de enzimas exógenas de tipo fibrolíticas sobre la composición química, consumo voluntario (CV), digestibilidad de nutrientes y degradabilidad in vitro aparente y verdadera de la materia seca (MS) de heno de pasto guinea (HPG)(Panicum Maximum Jacq.). Los productos enzimáticos utilizados fueron PromoteNET (Agribrand, Canadá) y BiocellulaseA20 (Loders Croklaan, Channahon, IL, USA), derivados de Trichoderma longibratum y Aspergillus reesei, conteniendo principalmente celulasas y xilanasas. En el primer experimento, nueve (9) carneros cruzados Blackbelly x criollo, de 26.8 Kg peso vivo promedio (PV) fueron utilizados en un diseño Cuadrado Latino 3x3 con tres periodos experimentales de 18 d de duración. Los tratamientos consistieron de heno sin aditivo (testigo), heno tratado con PromoteNET y heno tratado con BiocellulaseA-20, aplicado por rociada 24 h antes de ofrecerse. El ofrecimiento diario de heno fue al 4% del PV en base seca (BS). Los tratamientos enzimáticos aumentaron los contenidos de materia seca (MS) y proteína bruta (PB) en el HGP respecto al control (P< 0.05). Se observó una tendencia a reducir las concentraciones de fibra detergente neutro (FDN), fibra detergente ácido detergente (FDA) y hemicelulosa mediante los tratamientos enzimáticos, pero la misma no fue significativa (P< 0.05). El consumo voluntario (CV) de MS de los HPG tratados con BiocellulaseA-20 y PromoteNET (1139 y 938, g/d respectivamente) superaron al testigo (921 g/d). Los consumos de PB, FDN y FDA de HPG tratado con BiocellulaseA-20 fueron mayores (P< 0.05), que los de HPG sin aditivo o tratado con PromoteNET. El consumo diario de MS como porcentaje del PV fue mayor (P< 0.05) en corderos alimentados con HPG tratado con ambos productos enzimáticos respecto al testigo (4.06%, BiocellulaseA-20, 3.15% y PromoteNET vs. 2.86%). La digestibilidad aparente de la MS, aumentó (P> 0.05) entre los dos tratamientos enzimáticos, mientras que la digestibilidad de FDN fue mayor para HPG con BiocellulaseA-20. En el segundo experimento se encontró que las degradabilidades in vitro aparente (DIVMSA) y verdadera (DIVMSV) de la materia seca fueron mayores (P< 0.05) para el HPG tratado con ambas enzimas respecto al testigo (DIVMSA por 2.75 unidades porcentuales para BiocellulaseA-20 y por 2.12 para PromoteNET; DIVMSV por 2.42 y 1.73 unidades, respectivamente). Estos resultados indican que la aplicación de complejos enzimáticos fibrolíticos a henos de gramíneas tropicales de baja calidad, pueden mejorar la calidad nutritiva sustancialmente.
dc.language.isoesen_US
dc.subjectPanicum maximum Jacq.en_US
dc.subjectEnzimas exógenasen_US
dc.subjectHenoen_US
dc.subjectGuineaen_US
dc.titleEnzimas exógenas tipo fibroliticas sobre el consumo voluntario y digestibilidad de nutrientes de heno de pasto guinea (Panicum maximum Jacq.)en_US
dc.typeThesisen_US
dc.rights.licenseAll rights reserveden_US
dc.rights.holder(c) 2007 Karla M. Tous Riveraen_US
dc.contributor.committeeValencia Chin, Elide
dc.contributor.committeeSantiago Anadón, Héctor L.
dc.contributor.committeeRandel Folling, Paul
dc.contributor.committeeLatorre Acevedo, José R.
dc.contributor.representativeMás, Edwin
thesis.degree.levelM.S.en_US
thesis.degree.disciplineAnimal Industryen_US
dc.contributor.collegeCollege of Agricultural Sciencesen_US
dc.contributor.departmentDepartment of Animal Scienceen_US
dc.description.graduationYear2007en_US


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