Show simple item record

dc.contributor.advisorVargas-Ayala, Roberto
dc.contributor.authorArenas-Buitrago, Yolima A.
dc.date.accessioned2018-11-21T10:18:51Z
dc.date.available2018-11-21T10:18:51Z
dc.date.issued2014
dc.identifier.urihttps://hdl.handle.net/20.500.11801/1455
dc.description.abstractSoil samples were collected in four areas of Puerto Rico; three forest areas and one agricultural area cultivated with soybeans in order to isolate and identify antagonistic fungi to the soybean cyst nematode, Heterodera glycines (SCN). The fungi were isolated in semi-selective culture media using the serial dilutions of soil method. Fungi with nematode antagonistic characteristics were purified and retained in a culture medium of potato dextrose agar (PDA) with chitin. Four isolates of Paecilomyces lilacinus from soil samples collected in the forest area were identified; also a Trichoderma harzianum was isolated from samples of the area cultivated with soybeans. Subsequently, antagonism tests were conducted in the laboratory and greenhouse on eggs and juvenile larvae of SCN for three planting dates. To determine the degree of parasitism, the alginate films technique was used and fungal growth on cysts, larvae and eggs of the nematode was observed. In addition, greenhouse tests were conducted with soybean plants to examine the level of nematode control and the effect on plants. The results at both laboratory and greenhouse tests, showed antagonistic activity of five fungi on cysts, eggs, and larvae of SCN. Results showed that the morpho-species P. lilacinus had the highest incidence of parasitism on eggs and some effect on the larvae. The TN9P isolated from a forest area showed the best value regarding to results on the best the eggs parasitism. Fungi showed no negative effects on the development of soybean plants; in the case of T. harzianum and stimulation of the root growth was registered. These results show that isolated fungi have potential for use in a management program of SCN.en_US
dc.description.abstractCon el fin de aislar e identificar hongos con características antagónicas al nematodo del quiste de la soya, Heterodera glycines (NQS), se realizaron muestreos de suelo en cuatro áreas de Puerto Rico, tres zonas forestales y una zona agrícola cultivada con soya. Los hongos se aislaron en medios de cultivo semiselectivo siguiendo el método de diluciones seriadas de suelo. Los hongos que presentaron características antagónicas a nematodos se purificaron y conservaron en medio de cultivo de papa dextrosa agar (PDA) con quitina. Se identificaron cuatro aislados de Paecilomyces lilacinus procedentes de las muestras de suelo del área de reserva y un aislado de Trichoderma harzianum procedente de las muestras del área cultivada con soya. Posteriormente, se realizaron pruebas de antagonismo a nivel de laboratorio e invernadero sobre huevos y larvas J2 de NQS durante tres fechas de siembra. Para conocer el grado de parasitismo, se utilizó la técnica de láminas de alginato y se observó el desarrollo del hongo sobre quistes, larvas y huevos del nematodo. Además, se realizaron ensayos de invernadero con plantas de soya, para examinar el nivel de control sobre el nematodo y el efecto sobre las plantas. Los resultados tanto a nivel de laboratorio como en invernadero, mostraron actividad antagónica de los cinco hongos sobre quistes, huevos y larvas de NQS, donde las morfoespecies de P. lilacinus., presentaron una mayor incidencia de parasitismo sobre los huevos y una leve acción sobre las larvas. El aislado TN9P procedente de un área boscosa mostro los mejores resultados antagónicos sobre huevos en comparación con los otros aislados. Los hongos no presentaron efectos negativos sobre el desarrollo de las plantas de soya, por el contrario los tratamientos con T. harzianum estimularon su desarrollo radicular. Los resultados obtenidos demuestran que los hongos aislados tienen potencial para utilizarse en un programa de manejo de NQS.en_US
dc.description.sponsorshipProyecto TSTAR 131 de la Estación Experimental Agrícolaen_US
dc.language.isoesen_US
dc.subjectPaecilomyces lilacinusen_US
dc.subjectSoybean cyst nematodeen_US
dc.subjectAntagonistic fungien_US
dc.subjectBiological controlen_US
dc.subjectTrichoderma harzianumen_US
dc.titlePaecilomyces lilacinus (Thom) Samson y Trichoderma harzianum (Rifai) antagonistas del nematodo de quiste de la soya Heterodera glycines (Ichinohe) presentes en suelos de Puerto Ricoen_US
dc.typeThesisen_US
dc.rights.licenseAll rights reserveden_US
dc.rights.holder(c) 2014 Yolima Andrea Arenas Buitragoen_US
dc.contributor.committeeRivera Vargas, Lydia I.
dc.contributor.committeeValencia, Elide
dc.contributor.representativeRivera, Rosita
thesis.degree.levelM.S.en_US
thesis.degree.disciplineCrop Protectionen_US
dc.contributor.collegeCollege of Agricultural Sciencesen_US
dc.contributor.departmentDepartment of Crops and Agro-Environmental Sciencesen_US
dc.description.graduationSemesterSpringen_US
dc.description.graduationYear2014en_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

  • Theses & Dissertations
    Items included under this collection are theses, dissertations, and project reports submitted as a requirement for completing a graduate degree at UPR-Mayagüez.

Show simple item record

All rights reserved
Except where otherwise noted, this item's license is described as All Rights Reserved