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dc.contributor.advisorNadathur, Govind S.
dc.contributor.authorPérez-Guzmán, Lumarie
dc.description.abstractThe marine toxic dinoflagellate Ostreopsis lenticularis Fukuyo 1981 is implicated as the major vector in ciguatera fish poisoning in the southwest coast of Puerto Rico. Studies have demonstrated that bacteria play a role in the production of ciguatoxin by the dinoflagellate and that different clonal cultures of Ostreopsis harbor different culturable bacterial symbionts. It was recently demonstrated the presence of viable but non culturable (VBNC) species of bacteria associated with O. lenticularis. More than 100 associated bacteria from two toxic clonal cultures of Ostreopsis (#302, and #303), were examined for common bacterial strains that could be involved in the production of ciguatoxins. DNA extraction, PCR amplification of partial small subunit ribosomal DNA (rDNA), denaturing gradient gel electrophoresis (DGGE) and DNA sequencing were performed. One uncultured bacterium belonging to the genus Cytophaga was found to be common to both clones, indicating a close association with the dinoflagellate and its possible involvement in the ciguatoxin production.en_US
dc.description.abstractEl dinoflagelado tóxico marino Ostreopsis lenticularis es considerado el vector principal en envenenamiento por ciguatera en la costa suroeste de Puerto Rico. Estudios han demostrado que las bacterias llevan a cabo un rol en la producción de ciguatoxina por el dinoflagelado y que cada clon contiene diferentes simbiontes cultivables. Recientemente se demostró la presencia de bacterias viables pero no cultivables (VPNC) en Ostreopsis. Se estudiaron más de 100 bacterias asociadas con dos cultivos clonales tóxicos de O. lenticularis (#302 y #303) para identificar especies que puedan estar involucradas en la producción de la toxina. Se realizó extracción de ADN, amplificación parcial de la sub-unidad pequeña del ADN ribosomal, electroforesis de gel denaturalizante en gradiente y secuenciación del ADN. Una bacteria no-cultivada perteneciente al género Cytophaga fue encontrada común en ambos cultivos clonales de Ostreopsis. Esto indica una posible asociación de la bacteria con el dinoflagelado y sugiere que Cytophaga puede estar implicada en la producción de ciguatoxinas.en_US
dc.subjectOstreopsis lenticularis – Ciguatera fish poisoning vector - Puerto Ricoen_US
dc.subjectMarine toxic dinoflagellate – DNA synthesis - Puerto Ricoen_US
dc.subjectOstreopsis lenticularis – Ciguatoxin producer - Puerto Ricoen_US
dc.subject.lcshAlgal toxins--Toxicology.en_US
dc.subject.lcshToxic marine algae--Toxicology.en_US
dc.subject.lcshPoisonous fishes--Toxicology.en_US
dc.titleCommon bacterial symbionts in clonal cultures of the toxic dinoflagellate Ostreopsis lenticularisen_US
dc.rights.licenseAll rights reserveden_US
dc.rights.holder(c) 2006 Lumarie Pérez-Guzmánen_US
dc.contributor.committeeAponte, Nilda E.
dc.contributor.committeeBonaventura, Joseph
dc.contributor.committeeTosteson, Thomas R.
dc.contributor.representativeÁlvarez, Pedro Sciencesen_US
dc.contributor.collegeCollege of Arts and Sciences - Sciencesen_US
dc.contributor.departmentDepartment of Marine Sciencesen_US

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