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dc.contributor.advisorNadathur, Govind S.
dc.contributor.authorTirado Acevedo, Oscar
dc.date.accessioned2018-12-15T12:03:12Z
dc.date.available2018-12-15T12:03:12Z
dc.date.issued2004
dc.identifier.urihttps://hdl.handle.net/20.500.11801/1663
dc.description.abstractSeveral microorganisms have been isolated from different tropical ecosystems (tropical rain forest, sea water, and marine sediments) from the island of Puerto Rico. These microorganisms were screened for carbohydrase activity using chromogenic substrates (amylose, pullulan, cellulose, dextran, arabinan, galactomannan, and xylan). The results indicate that 2 - 9 % of cultured organisms show carbohydrase activity. Bacterial isolates were identified by sequence analysis of the PCR amplified partial subunit rRNA sequence. NCBI’s database comparison of the most versatile strains gives a match with Bacillus sp., Staphylococcus sp., Halomonas sp., and a gamma proteobacterium, respectively. Growth curves indicate that all strains assayed can grow as well in at least one complex carbohydrate as in glucose. We have detected a high β- xylosidase activity from our Bacillus sp. strain crude enzyme preparation. Identification from isolated fungi indicates they belong to genus Aspergillus and Microsporium respectively. A yeast isolate was identified by D1/D2 domain of the large subunit (26S) ribosomal DNA sequence; it gives a match with Glomus sp. Our results show that tropical environments have potentially good sources of microorganisms with novel carbohydrases.en_US
dc.description.abstractVarios microorganismos fueron aislados en ecosistemas tropicales diferentes por ejemplo: (bosque tropical lluvioso, agua de mar, y sedimentos marinos) de la isla de Puerto Rico. Estos microorganismos fueron seleccionados por su actividad de carbohidrasas usando sustratos cromogénicos (amilosa, pululano, celulosa, dextrano, arabinano, galactomanano, y xilano). Los resultados indican que el 2 - 9 % de los organismos cultivados muestran actividad de carbohidrasas. Las bacterias aisladas fueron identificadas mediante el análisis de la secuenciación parcial de la subunidad pequeña de rRNA amplificado mediante RPC. La comparación de las cepas más versátiles con la base de datos de NCBI nos da un resultado de identificación con: Bacillus sp., Staphylococcus sp., Halomonas sp., y gamma proteobacteria, respectivamente. Las curvas de crecimiento indican que todas las cepas ensayadas pueden crecer tan bien en al menos un carbohidrato complejo como en glucosa. Pudimos detectar una actividad alta de β-xylosidasa en la preparación de la enzima cruda de la cepa identificada como Bacillus sp. La identificación de los hongos aislados indica que pertenecen al género Aspergillus y Microsporium respectivamente. Una levadura aislada fue identificada mediante sequenciación del dominio D1/D2 de la subunidad grande (26S) de DNA ribosomal; el resultado da una comparación con Glomus sp. Nuestros resultados muestran que los ambientes tropicales tienen el potencial para buenas fuentes de microorganismos con actividad de carbohidrasas poco comunes.en_US
dc.description.sponsorshipNational Renewable Energy Laboratory (NREL)en_US
dc.language.isoenen_US
dc.subjectTropical environments – Microorganisms – Carbohydrases – Puerto Ricoen_US
dc.subjectMicroorganisms – Analysis – Effect of chromogenic bacteria on – Puerto Ricoen_US
dc.subject.lcshMicroorganisms--Biodegradation.en_US
dc.subject.lcshCarbohydrates--Biodegradation.en_US
dc.subject.lcshMarine ecology.en_US
dc.subject.lcshBiotic communities.en_US
dc.titleCharacterization of microorganisms with carbohydrase activities from tropical ecosystemsen_US
dc.typeThesisen_US
dc.rights.licenseAll rights reserveden_US
dc.rights.holder(c) 2004 Oscar Tirado Acevedoen_US
dc.contributor.committeeTosteson, Thomas R.
dc.contributor.committeeNieves, Rafael
dc.contributor.committeeAponte, Nilda
dc.contributor.representativeParés, Elsie I.
thesis.degree.levelM.S.en_US
thesis.degree.disciplineMarine Sciencesen_US
dc.contributor.collegeCollege of Arts and Sciences - Sciencesen_US
dc.contributor.departmentDepartment of Marine Sciencesen_US
dc.description.graduationSemesterSpringen_US
dc.description.graduationYear2004en_US


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