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dc.contributor.advisorVan Dam, Alex
dc.contributor.authorMassini Espino, Marlys
dc.date.accessioned2019-03-01T12:15:54Z
dc.date.available2019-03-01T12:15:54Z
dc.date.issued2018-12-10
dc.identifier.urihttps://hdl.handle.net/20.500.11801/1821
dc.description.abstractMolecules of DNA are ubiquitous in the environment and a rich source of biological information, but because DNA degrades rapidly in tropical climates studies of ancient DNA in the Caribbean have been limited until recently. Fossilized owl pellets are one potential source of ancient DNA with useful phylogenetic information. In this study, we obtained a fossilized Rattus rattus sample from an owl pellet excavated from Tres Bocas Cave in the Dominican Republic. We sequenced DNA pooled from three rat maxillae from the pellet, annotated the mitochondrial genome and compared the sequence to contemporary data from R. rattus from other studies. Maximum Likelihood phylogenetic analysis showed the sample from the Dominican Republic was nested within a clade of R. rattus from Madagascar and East Africa. The sequence from R. rattus from the Dominican Republic had few nucleotide misincorporations, suggesting a recent origin, which was corroborated with 14C isotope ratios that indicated the sample dates from 1960-70. We have demonstrated that phylogenetic information can be obtained from DNA from bones in decades-old pellets in a tropical region, which validates an ancient DNA extraction technique and indicates further sampling in the Caribbean may prove fruitful.en_US
dc.language.isoenen_US
dc.subjectancient DNAen_US
dc.subjectRattus rattusen_US
dc.subjectphylogenyen_US
dc.subjectmitochondrial genomeen_US
dc.subjecttropicsen_US
dc.subject.lcshMitochondrial DNA -- Analysisen_US
dc.subject.lcshRattus rattus -- Geneticsen_US
dc.subject.lcshRattus rattus -- Dominican Republic -- Phylogenyen_US
dc.subject.lcshRattus rattus -- Tres Bocas cave (Dominican Republic)en_US
dc.titleMitochondrial genome reconstruction of Rattus rattus obtained from an owl pellet from Tres Bocas Cave in the Dominican Republicsen_US
dc.typeThesisen_US
dc.rights.licenseAll rights reserveden_US
dc.rights.holder(c) 2018 Marlys Massini Espinoen_US
dc.contributor.representativeCruzado, Ivette
thesis.degree.levelM.S.en_US
thesis.degree.disciplineBiologyen_US
dc.contributor.collegeCollege of Arts and Sciences - Sciencesen_US
dc.contributor.departmentDepartment of Biologyen_US
dc.description.graduationSemesterFallen_US
dc.description.graduationYear2018en_US


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