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dc.contributor.advisorMaldonado Ramírez, Sandra L.
dc.contributor.authorVélez Rodríguez, Zuleimary
dc.date.accessioned2019-03-11T18:02:33Z
dc.date.available2019-03-11T18:02:33Z
dc.date.issued2018-11-30
dc.identifier.urihttps://hdl.handle.net/handle/20.500.11801/1842
dc.description.abstractAerobiology is responsible for studying atmospheric biological particulate such as pollen and fungal spores. Dispersion of this particulate is widespread, so its capture can be difficult, and, for this reason, different methods have been developed to achieve their capture. In this research a modified unmanned aerial vehicle, commonly known as drone, with spore traps was used to confirm it as an effective method for collecting spores and pollen from the lower atmosphere. Two areas with different anthropogenic activity, urban and rural, were chosen. In both, twelve samplings of 5 minutes flights were made, where the samplers were opened, and Petri dishes were exposed to collect viable particulate. Also, adhesive slides were exposed to collect non-viable particulate. Viable samples were cultivated, isolated and identified to observe diversity and frequency of the captured fungal genera. From non-viable samples, fungal spores and pollen, were observed directly under the microscope and identified. From the viable samples, 55 colonies were identified within 11 genera: Cladosporium, Curvularia, Fusarium, Penicillium, Aspergillus, Nigrospora, Pestalotia, Rhizopus, Colletotrichum, Paecilomyces and Leptosphaerulina. From the non-viable samples, 54 genera of fungal spores and 17 pollen genera were identified.en_US
dc.description.abstractLa aerobiología se encarga de estudiar el particulado atmosférico como el polen y las esporas de hongos. La dispersión de este particulado es amplia por lo que su captura puede ser difícil y para esto se han desarrollado distintos métodos para lograr su captura. En esta investigación se utilizó un vehículo aéreo manejado por control remoto, comúnmente conocido como dron, modificado con atrapadores de esporas para confirmarlo como un método efectivo de colecta de esporas y polen en la baja atmósfera. Se escogieron dos áreas con actividad antropogénica distinta, área urbana y área rural. En ambas se realizaron doce muestreos que consistían en vuelos de 5 minutos de duración donde se abrían los muestreadores y se exponían platos Petri con medio de cultivo para colectar particulado viable. También se expusieron laminillas adhesivas para colectar particulado no viable. Las muestras viables se cultivaron, aislaron e identificaron para observar diversidad y frecuencia de los géneros fúngicos capturados. Las muestras no viables, esporas de hongos y polen, fueron observadas directamente bajo microscopio e identificadas. De las muestras viables se lograron identificar 55 colonias de 11 géneros distintos: Cladosporium, Curvularia, Fusarium, Penicillium, Aspergillus, Nigrospora, Pestalotia, Rhizopus, Colletotrichum, Paecilomyces and Leptosphaerulina. De las muestras de particulado no viable se lograron identificar 54 géneros de esporas de hongos y 17 géneros de polen.en_US
dc.language.isoesen_US
dc.subjectAerobiologyen_US
dc.subjectFungal sporesen_US
dc.subjectPollenen_US
dc.subjectDronesen_US
dc.subjectLow atmosphereen_US
dc.subject.lcshAir -- Microbiologyen_US
dc.subject.lcshAir -- Pollutionen_US
dc.subject.lcshParticlesen_US
dc.subject.lcshFungi -- Sporesen_US
dc.subject.lcshPollen --Dispersalen_US
dc.titleUso de drones para la recuperación de esporas de hongos en la baja atmósferaen_US
dc.typeThesisen_US
dc.rights.licenseAll rights reserveden_US
dc.rights.holder(c) 2018 Zuleimary Vélez Rodríguezen_US
dc.contributor.committeeTorres Pratts, Hernán
dc.contributor.committeeCafaro, Matías
dc.contributor.representativePalomera, Rogelio
thesis.degree.levelM.S.en_US
thesis.degree.disciplineBiologyen_US
dc.contributor.collegeCollege of Arts and Sciences - Sciencesen_US
dc.contributor.departmentDepartment of Biologyen_US
dc.description.graduationSemesterFallen_US
dc.description.graduationYear2018en_US


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