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dc.contributor.advisorCafaro, Matías J.
dc.contributor.authorAponte-López, Carla M.
dc.date.accessioned2019-03-11T18:33:27Z
dc.date.available2019-03-11T18:33:27Z
dc.date.issued2018-05
dc.identifier.urihttps://hdl.handle.net/handle/20.500.11801/1856
dc.description.abstractCapsicum chinense is widely cultivated in the Caribbean region. Most types are pungent, such as 'Scotch Bonnet' and 'Habanero'. In Puerto Rico, non-pungent types called "ají dulce" are consumed as part of the local cuisine. Currently, in commercial agriculture, the use of chemical fertilizers dominates the local market, while biological ones are overlooked. The purpose of this research was to characterize and identify the endomycorrhizae associated with locally grown C. chinense in the western area of Puerto Rico. We collected “ají dulce” roots and surrounding soil from plants growing in experimental plots at UPRM. Plants were grown under six treatments: (1) commercial Promix® BX, (2) Promix® Mycorrhizae (Glomus intraradices), (3) Promix® mixed with 25% soil, (4) Promix® BX plus inorganic (N-P-K) fertilizer, (5) Promix® Mycorrhizae (Glomus intraradices) plus inorganic fertilizer and (6) Promix® mixed with 25% soil plus inorganic fertilizer. Aji dulce planted in Promix® Mycorrhizae without fertilizer had greater plant height, and a larger number of branches, leaves and fruits, compared to the other substrates. But when fertilizer was added, there was little or no positive response of plants in Promix Mycorrhizae. To determine the percentage mycorrhizae infection, slides were done utilizing Trypan blue-stained root cuts. Differences in percentage infection were found among treatments with Promix® + Soil exhibiting the highest percentage infection. DNA extractions and PCR reactions using specific primers for mycorrizhae were performed. Cloning of PCR products for sequencing were done. Based on GenBank database searches, we established that the genera Scutellospora, Diversispora, Acaulospora, Racocetra, Funneliformis, Glomus, and Paraglomus are present in local soils.en_US
dc.description.abstractCapsicum chinense es ampliamente cultivado en la región del Caribe. La mayoría de sus tipos son picantes como el 'Scotch Bonnet' y el 'Habanero'. En Puerto Rico, los tipos no picantes son llamados "ají dulce" y son consumidos como parte de la cocina local. Actualmente, en la agricultura comercial, el uso de los fertilizates químicos es lo que domina el mercado local, mientras que los biológicos son pasados por alto. El propósito de esta investigación es caracterizar e identificar las endomicorrizas asociadas a C. chinense crecido localmente en el área oeste de Puerto Rico. Se recolectaron raíces de “ají dulce” y suelo adyacente a las plantas creciendo en predios experimentales de UPRM. Estas plantas estuvieron expuestas a seis tratamientos usando (1) Promix® BX para uso general comercial, (2) Promix® Mycorrhizae (Glomus intraradices), (3) Promix® con 25% del suelo del invernadero de UPRM, (4) Promix® BX para uso general comercial con fertilizante, (5) Promix® Mycorrhizae (Glomus intraradices) con fertilizante y (6) Promix® con 25% del suelo del invernadero de UPRM con fertilizante. Aji dulce plantado en Promix® Mycorrhizae sin fertilizante tuvo mayor altura de la planta, cantidad de ramas, hojas y frutos comparado con los otros substratos. Pero cuando se añadia fertilizante había poca o ninguna respuesta positiva de las plantas con Promix® Mycorrhizae. Además, para calcular el porciento de infección, se hicieron laminillas utilizando cortes de raíces teñidas con Trypan blue. Por consiguiente, diferencias en el porcentaje de infección fueron encontradas entre los tratamientos donde Promix® + suelo con fertilizante obtuvo el porciento de infección más alto. Se realizaron extracciones de DNA y reacciones de PCR utilizando cebadores específicos para micorrizas. Se clonaron los productos de PCR y se secuenciaron. Usando búsquedas en GenBank el análisis sugiere la presencia en el suelo de UPRM de los siguientes géneros de micorrizas: Scutellospora, Diversispora, Acaulospora, Racocetra, Funneliformis, Glomus, y Paraglomus.en_US
dc.description.sponsorshipUSDA-NIFA-Hatch Accession No. 1000526 (H443)en_US
dc.language.isoenen_US
dc.subjectCapsicum chinense Jacquin - Puerto Ricoen_US
dc.subjectMycorrhizae - Symbiosis - Capsicum chinense Jacquin - Puerto Ricoen_US
dc.subjectSweet chili pepper - Puerto Ricoen_US
dc.subject.lcshCapsicum chinense--Inoculationen_US
dc.subject.lcshEndomycorrhizasen_US
dc.subject.lcshVesicular-arbuscular mycorrhizasen_US
dc.titleResponse of Capsicum chinense to mycorrhizae inoculation and local mycorrhizal diversity associated with the crop in Puerto Ricoen_US
dc.typeThesisen_US
dc.rights.licenseAll rights reserveden_US
dc.rights.holder(c) 2018 Carla Michelle Aponte Lópezen_US
dc.contributor.committeeBeaver, Linda Wessel
dc.contributor.committeeRíos Velázquez, Carlos
dc.contributor.committeeVélez, Ana
dc.contributor.representativeMedina Díaz, César A.
thesis.degree.levelM.S.en_US
thesis.degree.disciplineBiologyen_US
dc.contributor.collegeCollege of Arts and Sciences - Sciencesen_US
dc.contributor.departmentDepartment of Biologyen_US
dc.description.graduationSemesterSpringen_US
dc.description.graduationYear2018en_US


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