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dc.contributor.advisorCafaro, Matías J.
dc.contributor.authorFigueroa-Negrón, Verónica
dc.date.accessioned2019-03-11T18:35:01Z
dc.date.available2019-03-11T18:35:01Z
dc.date.issued2018-05
dc.identifier.urihttps://hdl.handle.net/handle/20.500.11801/1867
dc.description.abstractThe search for new antimicrobial compounds has been of great interest in recent years. These compounds are naturally produced by microorganisms including endophytic fungi isolated from mangroves. This study focuses on endophytic fungi isolated from leaves and seeds of the black mangrove (Avicennia germinans) from Bahía Salinas, Cabo Rojo, Puerto Rico. We isolated 20 genera of fungal endophytes from which nine identified strains were tested for antimicrobial secondary metabolite production. Fungal strains from Penicillium (BSI-HV-1(2)) sp., Aspergillus flavus (BSI-HV-2(1)), Aspergillus clavatus (BSI(MH)-HJ-2(1)), Stereum (BSI-R-2(1)) sp., Nigrospora (Khuskia oryzae) (BSI(1.5)-HJ-2(1)), Purpureocillium (BSII(3.5)-R-3(2)) sp., Simplicillium (BSI(1.5)-R-1(1)) sp., Hortaea (BSII(3.5)-HC-1(2)) sp. and Bionectria (BSI(MH)-HC-3(1)) sp. were selected for analysis. Four bacterial strains: Escherichia coli, Pseudomonas sp., Serratia sp. and Staphylococcus sp. and two yeast strains of Candida albicans and C. tropicalis were used to perform bioassays (ATCC certified strains). Growth curves were prepared for yeasts and bacteria. Fungal extracts were added after lag, exponential and stationary growth phases of each bacteria and yeast culture. Our results showed that A. flavus (BSI-HV-2(1)) and A. clavatus (BSI(MH)-HJ-2(1)) extracts strongly decreased the growth of C. albicans, P. aeruginosa, and E. coli. Only A. flavus (BSI-HV-2(1)) decreased the growth of C. tropicalis. Stereum (BSI-R-2(1)) sp. enhanced the growth of E. coli and A. flavus (BSI-HV-2(1)) enhanced the growth of S. aureus. These results could open a way for future discoveries of new antimicrobial or antifungal compounds from the black mangrove endophytes in Puerto Rico.en_US
dc.description.abstractLa búsqueda de nuevos compuestos antimicrobiales ha sido de gran interés durante los últimos años. Estos compuestos pueden ser producidos naturalmente por muchos organismos, entre ellos, hongos aislados de mangles. Este estudio se enfoca en los hongos endófitos aislados de las hojas y semillas del mangle negro (Avicennia germinans) en Bahía Salinas, Cabo Rojo, Puerto Rico. Aislamos 20 géneros de hongos endófitos de los cuales se escogieron 9 para determinar si los metabolitos secundarios producidos tenían alguna capacidad antimicrobial. Las especies de hongos utilizadas fueron Penicillium (BSI-HV-1(2)) sp., A. flavus (BSI-HV-2(1)), Aspergillus clavatus (BSI(MH)-HJ-2(1)), Stereum (BSI-R-2(1)) sp., Nigrospora (Khuskia oryzae) (BSI(1.5)-HJ-2(1)), Purpureocillium (BSII(3.5)-R-3(2)) sp., Simplicillium (BSI(1.5)-R-1(1)) sp., Hortaea (BSII(3.5)-HC-1(2)) sp. y Bionectria (BSI(MH)-HC-3(1)) sp. Utilizamos cuatro cepas de bacterias para realizar los bioensayos: Escherichia coli, Pseudomonas sp., Serratia sp. y Staphylococcus sp., así como dos cepas de levaduras, Candida albicans y C. tropicalis (cepas certificadas ATCC). Se realizaron curvas de crecimiento para determinar las fases de crecimiento de cada bacteria y levadura. Luego se añadió el sobrenadante del hongo (extractos) para ver cómo los metabolitos secundarios excretados por el mismo afectaban la curva normal de crecimiento en las fases lag, exponencial y estacionaria de cada espécimen. Se encontró que los extractos de A. flavus (BSI-HV-2(1)) y A. clavatus (BSI(MH)-HJ-2(1)) disminuyeron el crecimiento de C. albicans, P. aeruginosa y E. coli. Sólo A. flavus (BSI-HV-2(1)) disminuyó el crecimiento de C. tropicalis, mientras que los extractos de Stereum (BSI-R-2(1)) sp. y A. flavus (BSI-HV-2(1)) aumentaron el crecimiento de E. coli y S. aureus, respectivamente. Estos resultados abren nuevas puertas a posibles descubrimientos de nuevos productos antimicrobiales provenientes de hongos endófitos de mangle negro en Puerto Rico.en_US
dc.description.sponsorshipBRIDGES to Doctorate Program (Grant number R25GM058389)en_US
dc.language.isoenen_US
dc.subjectMangrove fungal endophytes - Study of - Antimicrobial, antifungal and anticarcinogenic properties - Puerto Ricoen_US
dc.subjectBlack mangrove (Avicennia germinans) - Endophytic fungi - Cabo Rojo, P.R.en_US
dc.subjectBlack mangrove (Avicennia germinans) - Leaves - Antimicrobial compounds - Cabo Rojo, P.R.en_US
dc.subject.lcshEndophytic fungi--Puerto Rico--Cabo Rojoen_US
dc.subject.lcshPlant-fungus relationshipsen_US
dc.subject.lcshAnti-infective agentsen_US
dc.titleEndophytic fungi associated with the black mangrove Avicennia germinans in Cabo Rojo, Puerto Rico: Their antimicrobial potentialen_US
dc.typeThesisen_US
dc.rights.licenseAll rights reserveden_US
dc.rights.holder(c) 2018 Verónica Figueroa-Negrónen_US
dc.contributor.committeeRíos Velázquez, Carlos
dc.contributor.committeeMaldonado Ramírez, Sandra L.
dc.contributor.committeeVélez Díaz, Ana
dc.contributor.representativeCurbelo, Jaime E.
thesis.degree.levelM.S.en_US
thesis.degree.disciplineBiologyen_US
dc.contributor.collegeCollege of Arts and Sciences - Sciencesen_US
dc.contributor.departmentDepartment of Biologyen_US
dc.description.graduationSemesterSpringen_US
dc.description.graduationYear2018en_US


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