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dc.contributor.advisorResto-Irizarry, Pedro J.
dc.contributor.authorAgostini-Infanzon, Reinaldo J.
dc.date.accessioned2019-03-29T12:58:00Z
dc.date.available2019-03-29T12:58:00Z
dc.date.issued2018-12-12
dc.identifier.urihttps://hdl.handle.net/handle/20.500.11801/1882
dc.description.abstractTriple Negative Breast Cancer (TNBC) is distinguished by various characteristics which are associated to its poor prognosis. This type of cancer is aggressive in nature and has no targeted therapies. The tumor microenvironment has shown to modulate tumor cell behavior and response to clinical therapies. Current in vitro technologies allow us to observe multicellular interactions for extended periods; however, these present a variety of limitations including the need for cell sorting, requirement of large amounts of cells, and lack of tunable discrete adjacent compartments. Other technologies such as microfluidic platforms lack a user-friendly interface and require specialized instrumentation and training, making it difficult to be routinely used in a cell biology laboratory. To overcome these problems, an open multi-microwell triculture device was manufactured to probe tumor-stromal interactions. The devices were printed out of polystyrene sheets using a razor-blade plotter and manually layered into a 3-dimensional structure. We have evaluated the effect of normal and cancerous stromal cell clusters (fibroblasts and macrophages) in modulating tumor behavior in MDA-MB-231 cells. Tumor cell proliferation was evaluated at 72hrs cultured with stromal clusters under exposure to stress conditions. Results for normal stromal cell clusters show no significant difference in proliferation at 37°C and 41°C. On the other hand, cancer associated cells show an increased proliferation rate of 11.7% for 37°C than for 41°C. Normal stroma shows potential damping on the effects of the high temperature stress on the cells, as no significant difference was observed. Thus, normal stroma improves recovery from heat damage as compared to cancer-stroma. Multi-adjacent microwell stickers are a fast prototyping culture platform and provide flexibility for testing diverse biomaterials with varying conditions for each cell type in multi-culture cell signaling studies.en_US
dc.description.sponsorshipInvestigation subsidized by NSF CREST Grant No. HRD 1345156.en_US
dc.language.isoenen_US
dc.subjectMulti-cellular modelen_US
dc.subjectMagnetic fluid hyperthermiaen_US
dc.subjectMacrophagesen_US
dc.subjectTriple negative breast canceren_US
dc.subjectRazor cutteren_US
dc.subject.lcshNanoparticlesen_US
dc.subject.lcshBreast -- Canceren_US
dc.subject.lcshBiomedical materialsen_US
dc.subject.lcshCancer cellsen_US
dc.titleDevelopment and characterization of a multi-cellular in vitro model for the study of nanoparticle-cell Interfaceen_US
dc.typeThesisen_US
dc.rights.licenseAll rights reserveden_US
dc.rights.holder(c) 2018 Reinaldo Agostini Infanzónen_US
dc.contributor.committeeDomenech, Maribella
dc.contributor.committeeDíaz, Rubén
dc.contributor.representativePerales, Oscar
thesis.degree.levelM.S.en_US
thesis.degree.disciplineMechanical Engineeringen_US
dc.contributor.collegeCollege of Engineeringen_US
dc.contributor.departmentDepartment of Mechanical Engineeringen_US
dc.description.graduationSemesterFallen_US
dc.description.graduationYear2018en_US


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