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dc.contributor.advisorDe Jesús Ruiz, Marco A.
dc.contributor.authorGrecia V. Gratacós-Velázquez
dc.date.accessioned2019-04-15T13:45:16Z
dc.date.available2019-04-15T13:45:16Z
dc.date.issued2018
dc.identifier.urihttps://hdl.handle.net/handle/20.500.11801/1961
dc.description.abstractDespite recent advances, there is still a need to improve existing technology to achieve faster, more efficient identification tools for bacterial detection and monitoring. Raman scattering is information-rich vibrational spectroscopy that shows promise for bacterial monitoring and identification. The integration of Surface Enhanced Raman Scattering methods circumvents low sensitivity issues of conventional Raman by augmenting its effective cross-section, amplifying the observed signal responses. This work employs Silver-Polydimethylsiloxane nanocomposites as Raman substrates for effective identification of L. acidophilus, L. casei and E. coli as model probiotic and bacterial agents. The developed assay enables effective identification of characteristic Raman vibrations for L. casei at 740 cm-1, 860 cm-1, 1006 cm-1, 1031 cm-1 and 1610 cm-1, as well as the 1171 cm-1, 1245 cm-1, 1366 cm-1 and 1590 cm-1, for E. coli. The developed procedure shows a promising alternative to implement SERS based methods on bacteriological analyses in the food industry.en_US
dc.description.abstractA pesar de los avances recientes, aún existe la necesidad de mejorar las tecnologías existentes para la identificación eficiente de presencia de bacterias en los productos comerciales. La espectroscopía mediante la dispersión de luz, mejor conocido como espectroscopía Raman, ha demostrado ser una técnica prometedora para el monitoreo e identificación de agentes bioactivos que puedan estar presentes. Integrando SERS (Surface Enhanced Raman Scattering por sus siglas en inglés) se evita problemas de sensitividad baja al aumentar la sección cruzada de la dispersión Raman. Este trabajo emplea el uso de polidimetilsiloxano como sustrato para formar un nano-composito con plata para utilizarlo como sustrato de Raman en la identificación de L. acidophilus, L. casei y E. coli como modelo de agentes probióticos o bioactivos. Algunas vibraciones Raman características observadas para L. casei son 740 cm-1, 860 cm-1, 1006 cm-1, 1031 cm-1 and 1610 cm-1, as well as the 1171 cm-1, 1245 cm-1, 1366 cm-1 y 1590 cm-1, para E. coli. Este procedimiento desarrollado muestra ser una alternativa prometedora para implementar métodos basados en SERS para la identificación y caracterización rápida de bacterias en la industria de alimentos.en_US
dc.language.isoEnglishen_US
dc.subjectNanocompositesen_US
dc.titleMetal-polymer nanocomposites as raman probes for the identification of model probiotics, gram-negative and gram- positive bioactive agentsen_US
dc.typeThesisen_US
dc.rights.licenseAll rights reserveden_US
dc.rights.holder(c) 2018 Grecia V. Gratacós-Velázquezen_US
dc.contributor.committeeRivera-Portalatín, Nilka M.
dc.contributor.committeeSantana-Vargas, Alberto
dc.contributor.representativeOrtiz, Gloribell
thesis.degree.levelM.S.en_US
thesis.degree.disciplineChemistryen_US
dc.contributor.collegeCollege of Arts and Sciences - Sciencesen_US
dc.contributor.departmentDepartment of Chemistryen_US
dc.description.graduationSemesterSummer (3rd Semester)en_US
dc.description.graduationYear2018en_US


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    Items included under this collection are theses, dissertations, and project reports submitted as a requirement for completing a degree at UPR-Mayagüez.

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