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dc.contributor.advisorLópez-Garriga, Juan
dc.contributor.authorRamos-Álvarez, Cacimar A.
dc.description.abstractLucina pectinata (Gmelin, 1791) is a clam that can be found in sulfide-rich muds in the coastal mangroves of the southwest coast of Puerto Rico. It contains three different hemoglobins in the gill tissue, hemoglobin I, II and III (HbI, HbII and HbIII, respectively), each one characterized by distinct physical-chemical properties. Hemoglobin I (HbI) is a monomeric protein of 142 amino acid residues, and it is responsible for oxygen and/or hydrogen sulfide transportation. These proteins present an excellent scenario for the study of dynamics of their reaction with diatomic small molecules and H2S. With the development of a state-of-the-art ultrafast laser system and experimental setup we were able to study the initial steps in the reaction of HbI and HbI mutants in various electronic states with its native ligand (H2S) in the fs to ps time regime. The HbI-H2S kinetics showed a second component of ~12ps, two times faster than the same component for the metaquo specie. Furthermore the transient absorption spectra do not show the classical ligated to unligated trace indicating the presence of at least three different species during the initial photolysis steps. It has been observed that in the presence of H2S HbI can undergo reduction from its FeIII state, the mechanism is still unknown but our experiments demonstrate that this could be feasible. Measurements taken for the rHbI ferrous complex, demonstrated that small fraction of photo-oxidation of the sample occurred.en_US
dc.description.sponsorshipThe Science on Wheels Educational Centeren_US
dc.subjectLucina pectinataen_US
dc.subjectDiatomic small moleculesen_US
dc.subjectRecombinant hemoglobin Ien_US
dc.subjectFemtosecond spectroscopyen_US
dc.titleFemtosecond spectroscopy studies of recombinant hemoglobin I of the clam Lucina pectinata (Gmelin, 1791)en_US
dc.rights.licenseAll rights reserveden_US
dc.rights.holder(c) 2011 Cacimar A. Ramos Álvarezen_US
dc.contributor.committeeCastro, Miguel E.
dc.contributor.committeeHernández, Samuel
dc.contributor.committeeLópez, Gustavo
dc.contributor.representativeAponte, Nilda E. Chemistryen_US
dc.contributor.collegeCollege of Arts and Sciences - Sciencesen_US
dc.contributor.departmentDepartment of Chemistryen_US

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