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dc.contributor.advisorSiritunga, Dimuth
dc.contributor.authorHernández Rosario, Lumariz
dc.date.accessioned2019-06-14T13:23:08Z
dc.date.available2019-06-14T13:23:08Z
dc.date.issued2019
dc.identifier.urihttps://hdl.handle.net/20.500.11801/2469
dc.description.abstractThe Solanaceae family is one of the largest and well distributed plant families in the world. It contains species with agricultural and economical importance such as potatoes, eggplants, tomatoes and peppers. In Puerto Rico there are approximately 46 species of Solanaceae of which six are endemic: Brunfelsia densifolia, B. lactea, B. portoricensis, Goetzea elegans, Solanum ensifolium and S. woodburyi. The objective of this project was to use DNA barcoding to identify the Solanaceae species in Puerto Rico, including the endemics, and to assess the genetic relationships between them. To this end, two chloroplast regions (psbA-trnH and matK) and a nuclear region (ITS) were PCR- amplified, sequenced and submitted to comparative sequence analysis. Phylogenetic trees of single and concatenated regions were generated from sequences from different taxa obtained in this study and from deposited species in GenBank database. Phylogenetic analysis demonstrated that DNA barcoding can be used to discriminate at the species level among these taxa in Puerto Rico. For all three markers, the genus that showed the highest pairwise distance specific value between represented species was Solanum, while the genus that displayed the least was Capsicum with 0.000 p-distance value. The results show that this technique can be used to identify species with one, two, or three combinations of DNA barcode markers depending on the taxon. In addition, this is the first study to include the endemic species Solanum woodburyi in a molecular phylogenetic analysis, and it was found to have a close relationship with S. ensifolium, also endemic to Puerto Rico, and to S. bahamense from the Bahamas and Greater Antilles. Therefore, we suggest the inclusion of Solanum woodburyi as part of the Bahamense clade.en_US
dc.description.abstractLa familia Solanaceae es una de las familias más grandes y de distribución a nivel mundial. Dentro de esta familia se encuentran especies de gran importancia agrícola y económica como la papa, la berenjena, el tomate y los pimientos. En Puerto Rico hay aproximadamente 46 especies descritas de Solanaceae de las cuales seis son especies endémicas: Brunfelsia densifolia, B. lactea, B. portoricensis, Goetzea elegans, Solanum ensifolium y S. woodburyi. El objetivo de este estudio es probar si la técnica de código de barras de ADN puede ser utilizada como una forma fácil y rápida de identificar estas especies; a la vez nos interesa estudiar la relación entre especies que existe entre estas. Para llevar a cabo este estudio dos regiones de cloroplasto y una nuclear ya determinadas como regiones de código de barras de ADN fueron amplificadas: psbA-trnH, matk y ITS respectivamente. En total se estudiaron el 76% de las especies de Solanaceae de Puerto Rico. Se realizaron análisis de diferencias genéticas y arboles filogenéticos. Análisis de árboles filogenéticos de secuencias simples y concatenadas se generaron con muestras de este estudio y con la de la base de datos NCBI. Por otro lado, este es el primer estudio molecular que incluye a la especie S. woodburyi y encontramos una relación genética cercana a las especies S. ensifolium y S. bahamense. Por tal razón, sugerimos que S. woodburyi puede ser parte del clado Bahamense, clado que incluye a S. ensifolium y S. woodburyi.en_US
dc.description.sponsorshipThis project was supported by a grant to Dr. Dimuth Siritunga from USDA HIS program (2016-06469).en_US
dc.language.isoEnglishen_US
dc.subjectSolanaceae family
dc.subjectDNA barcoding
dc.subject.lcshSolanaceae -- Puerto Rico
dc.subject.lcshSolanaceae -- Genetics
dc.subject.lcshDNA -- Analysis
dc.subject.lcshPhylogeny
dc.subject.lcshPlant genetics -- Puerto Rico
dc.titleDNA barcoding of the Solanaceae family in Puerto Rico including endangered and endemic speciesen_US
dc.typeThesisen_US
dc.rights.licenseAll rights reserveden_US
dc.rights.holder(c) 2019 Lumariz Hernández Rosarioen_US
dc.contributor.committeeRodríguez Minguela, Carlos M.
dc.contributor.committeeVan Ee, Benjamin
dc.contributor.representativeSegarra Carmona, Alejandro E.
thesis.degree.levelM.S.en_US
thesis.degree.disciplineBiologyen_US
dc.contributor.collegeCollege of Arts and Sciences - Sciencesen_US
dc.contributor.departmentDepartment of Biologyen_US
dc.description.graduationSemesterSpring (2nd semester)en_US
dc.description.graduationYear2019en_US


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