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dc.contributor.advisorCafaro, Matías J.
dc.contributor.authorRiascos-Cuero, Carolina
dc.date.accessioned2018-02-15T19:30:37Z
dc.date.available2018-02-15T19:30:37Z
dc.date.issued2014
dc.identifier.urihttps://hdl.handle.net/20.500.11801/257
dc.description.abstractEl éxito ecológico de muchos insectos en áreas tales como la reproducción, la defensa contra patógenos y el suministro de nutrientes, se relaciona con simbiosis con bacterias del género Streptomyces (Actinobacteria). Estudios anteriores encontraron una comunidad específica de Streptomyces asociadas a nidos de Nasutitermes costalis. Esta investigación se basa en determinar la diversidad de la comunidad de Streptomyces en dos especies de termitas comunes en Puerto Rico: N. costalis y N. acajutlae y en tres zonas ecológicas: bosque húmedo, bosque seco y manglar. Se aislaron 1,666 colonias, de las cuales se determinaron 603 morfotipos macroscópicamente y 13 microscópicamente. Se analizaron las cepas obtenidas mediante la técnica de BOX-PCR y se construyó un dendrograma que mostró 42 grupos; el 57% de ellos contienen cepas que estaban relacionados con las tres zonas ecológicas y las dos especies de termitas. El otro 43% sólo se relaciona con una zona específica o una especie de termita específica. El análisis filogenético de las secuencias del gen 16S rRNA confirmó que las cepas aisladas pertenecen al género Streptomyces. Los índices de diversidad mostraron una comunidad diversa de Streptomyces para cada zona ecológica y cada especie de termita; no hay un grupo o cepa de Streptomyces dominando la comunidad en los nidos de estas termitas. También se evaluó la capacidad de las cepas de Streptomyces de degradar celulosa y se realizó un análisis de micronutrientes del nido con el fin de determinar un posible papel nutricional. El 90% de las cepas aisladas tienen la capacidad de degradar la celulosa. Los nutrientes Ca, K, Mg y NH4+ difieren en los nidos de ambas especies de termitas explicando la variación que se observa entre los nidos. Esta investigación es el primer estudio que identifica las cepas aisladas de Streptomyces de los nidos de dos especies diferentes de termitas arbóreas en tres diferentes zonas ecológicas de Puerto Rico.
dc.description.abstractThe ecological success of many insects in their reproduction, defense against pathogens and nutrient supply is related to symbiotic relationships with bacteria in the genus Streptomyces (Actinobacteria). Previous studies have established that a specific community of Streptomyces was associated with nests of Nasutitermes costalis. This research focused on the diversity of the community of Streptomyces in two species of termites common in Puerto Rico: N. costalis and N. acajutlae in three ecological zones: rainforest, dry forest and mangrove. A total of 1666 colonies were isolated; and 603 macroscopically and 13 microscopically morphotypes were determined. Isolated strains were analyzed with the BOX-PCR technique and a dendrograma showing 42 groups was constructed; 57% of these groups contain isolated strains that were recovered from the three ecological zones and both species of termites. The remaining 43% of the groups were associated with a specific zone or with a specific termite species. Phylogenetic analysis of the 16S rRNA confirmed that the isolated strains belong to the genus Streptomyces. The diversity indices analysis showed a diverse community of Streptomyces for each ecological zone and each termite species; however, no group of Streptomyces dominates the community. In addition, cellulose degradation capacity of selected strains and micronutrient composition of the nest were evaluated in order to determine a possible nutritional role. The results determined that 90% of the isolated strains have the ability to degrade cellulose. Nutrients differ between the nests of the two termite species and Ca, K, Mg and NH4+ drive the variation between them. This investigation is the first to identify isolated strains of Streptomyces from the nests of two different species of arboreal termites in three different ecological zones of Puerto Rico.
dc.description.sponsorship“National Science Foundation” (NSF)en_US
dc.language.isoesen_US
dc.subjectinsectosen_US
dc.subjectStreptomyces (Actinobacteria)en_US
dc.subjectsimbiosisen_US
dc.subjectNasutitermes costalisen_US
dc.subjectN. costalisen_US
dc.subjectN. acajutlaeen_US
dc.subjectBOX-PCRen_US
dc.subjectgen 16S rRNAen_US
dc.subject.lcshTermitidae--Nests--Puerto Ricoen_US
dc.subject.lcshTermites--Host plants--Puerto Ricoen_US
dc.subject.lcshStreptomyces--Puerto Ricoen_US
dc.subject.lcshEcological zones--Puerto Ricoen_US
dc.titleStreptomyces asociados a nidos de Nasutitermes costalis y Nasutitermes acajutlae (Isoptera: Termitidae) en tres zonas ecológicas de Puerto Ricoen_US
dc.typeThesisen_US
dc.rights.licenseAll rights reserveden_US
dc.rights.holder(c) 2014 Carolina Riascos Cueroen_US
dc.contributor.committeeMontalvo Rodríguez, Rafael
dc.contributor.committeeRodríguez Minguela, Carlos
dc.contributor.representativeVargas, Roberto
thesis.degree.levelM.S.en_US
thesis.degree.disciplineBiologyen_US
dc.contributor.collegeCollege of Arts and Sciences - Sciencesen_US
dc.contributor.departmentDepartment of Biologyen_US
dc.description.graduationSemesterSpringen_US
dc.description.graduationYear2014en_US


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