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dc.contributor.advisorMaldonado-Ramírez, Sandra L.
dc.contributor.authorMalavé-Vega, Selimar
dc.date.accessioned2018-05-16T16:36:14Z
dc.date.available2018-05-16T16:36:14Z
dc.date.issued2006
dc.identifier.urihttps://hdl.handle.net/20.500.11801/597
dc.description.abstractEndophytic fungi live inside plant tissues without any external manifestation. As reported previously, they play an important role in the vegetal tissues they inhabit. A study of the fungal endophytes of Thalassia testudinum in Playa Buyé, Cabo Rojo, Puerto Rico, was conducted to isolate and identify the cultivable micelial endophytes associated to healthy leaves of this seagrass, which is the most common and abundant in the Caribbean. The plant serves as habitat for marine organisms and as food for marine turtles. Two samplings were performed: one in July and another in December 2004. Leaves were surface sterilized in 0.5% sodium hypochlorite solution and rinsed twice in distilled water. Fragments of the sterilized leaves (apex, center and base) were placed in Marine Agar and incubated for a week at 25°C. Aspergillus fumigatus (38.3%), Curvularia brachyspora (12.8%), Aspergillus flavus (10.6%), Cladosporium oxysporum (10.6%), Fusarium sp. (8.5%), Acremonium sp. (4.3%), Cladosporium sp. (2.1%), Cladosporium cladosporioides (2.1%), Curvularia pallescens (2.1%), Penicillium janthinellum (2.1%), Aspergillus ustus (2.1%), Botrytis sp. (2.1%) and an unidentified morphotype (2.1%) were isolated. This is the first report of micelial endophytes associated to healthy leaves of T. testudinum in Puerto Rico. Aspergillus fumigatus, Aspergillus ustus and Cladosporium oxysporum are reported as endophytes for the first time.
dc.description.abstractLos hongos endofitos crecen internamente en los tejidos vegetales sin manifestación externa alguna. Estudios previos demuestran que éstos tienen un rol importante en los tejidos vegetales que habitan. Se realizó un estudio sobre hongos endofitos asociados a Thalassia testudinum en Playa Buyé, Cabo Rojo, Puerto Rico, para aislar e identificar endofitos miceliales cultivables en hojas sanas de esta hierba marina, que es la más común y abundante en el Caribe. La planta sirve de hábitat para organismos marinos y es fuente de alimentos para las tortugas marinas. Se realizaron dos muestreos, uno en julio y otro en diciembre 2004. Las hojas fueron esterilizadas en una solución de hipoclorito de sodio (0.5%) y enjuagues con agua destilada. Los fragmentos de tejido esterilizado (ápice, centro y base) se colocaron en Agar Marino y se incubaron a 25°C por una semana. Se aisló a Aspergillus fumigatus (38.3%), Curvularia brachyspora (12.8%), Aspergillus flavus (10.6%), Cladosporium oxysporum (10.6%), Fusarium sp. (8.5%), Acremonium sp. (4.3%), Cladosporium sp. (2.1%), Cladosporium cladosporioides (2.1%), Curvularia pallescens (2.1%), Penicillium janthinellum (2.1%), Aspergillus ustus (2.1%), Botrytis sp. (2.1%) y un morfotipo no identificado (2.1%). Este es el primer informe sobre hongos endofitos miceliales cultivables asociados a hojas sanas de T. testudinum en Puerto Rico. Aspergillus fumigatus, Aspergillus ustus y Cladosporium oxysporum son reportados como endofitos por primera vez.
dc.description.sponsorshipDepartamento de Recursos Naturales y Ambientales (permiso de investigación científica DRNA: 04-IC-045) para la colección de T. testudinum en Playa Buyé, Cabo Rojo, P.R.en_US
dc.language.isoesen_US
dc.subjectHongos endofitosen_US
dc.subjectThalassia testudinumen_US
dc.subjectEndofitos micelialesen_US
dc.subject.lcshEndophytic fungi--Seasonal variations--Puerto Rico--Cabo Rojoen_US
dc.subject.lcshTurtle grass--Puerto Rico--Cabo Rojo--Diseases and pests--Identificationen_US
dc.subject.lcshPlant-fungus relationshipsen_US
dc.titleCatastro de hongos endofitos miceliales cultivables en hojas de la hierba marina Thalassia testudinum en playa Buyé, Cabo Rojo, Puerto Ricoen_US
dc.typeThesisen_US
dc.rights.licenseAll rights reserveden_US
dc.rights.holder(c) 2006 Selimar Malavé Vegaen_US
dc.contributor.committeeMuñoz, Carlos A.
dc.contributor.committeeSantos, Carlos J.
dc.contributor.representativeZapata, Mildred
thesis.degree.levelM.S.en_US
thesis.degree.disciplineBiologyen_US
dc.contributor.collegeCollege of Arts and Sciences - Sciencesen_US
dc.contributor.departmentDepartment of Biologyen_US
dc.description.graduationYear2006en_US


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