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dc.contributor.advisorSiritunga, Dimuth
dc.contributor.authorLuciano-Rosario, Dianiris
dc.description.abstractNative to Central America, papaya (Carica papaya) is one of the most cultivated fruit crops in tropical areas of the world. The commercial success of papaya is not only due to its high nutritional qualities but also due of its short generation time. Assessing the genetic diversity of papaya is an important aspect of conservation of this important plant genetic resources. However, knowledge on the genetic diversity of papaya in Puerto Rico is poorly understood. Therefore, 139 papaya accessions collected from all over Puerto Rico were evaluated using 23 Simple Sequence Repeat (SSR) markers and compared to 13 varieties from the USDA repository and 10 commercial varieties that served as references. A total of 214 alleles were identified having a mean observed heterozygosity (Ho) of 0.219. The Inbreeding coefficient (F) yielded a value of 0.565 and when evaluating the population structure of these accessions, 2 groups (k=2) were identified. An Unweighted Pair Group Method with Arithmetic Mean (UPGMA) dendogram showed no geographical organization within the unknown Puerto Rico samples. Moreover, Single Nucleotide Polymorphisms (SNPs) identification using Genotyping by Sequencing was also used to assess the genetic diversity of the same samples. We found a total of 4, 245 SNPs. A mean observed heterozygosity (Ho) of 0.226 and Inbreeding Coefficient (Gis) of 0.067 was recorded. In agreement with the SSR analyses, the population structure showed that the samples grouped in 2 clusters (k=2). Overall, this study contributes to the knowledge of papaya genetic diversity in the Caribbean region which will be useful for the conservation of papaya genetic resources.en_US
dc.description.abstractLa papaya (Carica papaya), nativa de centro América, es uno de los cultivos frutales mas sembrados en las zonas tropicales del mundo. El éxito comercial de papaya no solo se debe a su alto valor nutritivo pero también recae en su corto tiempo de generación. Los análisis de diversidad genética componen un aspecto importante en la conservación de recursos fitogenéticos. Existe poco conocimiento acerca de la diversidad genética de papaya en Puerto Rico. Es por esto que se evaluaron 162 accesiones de papaya utilizando 23 microsatelites. De estas 162 accesiones, 139 son muestras desconocidas de Puerto Rico, 13 muestras del repositorio de USDA y 10 variedades comerciales. Se identificó un total de 214 alelos y una heterocigosidad observada promedio (Ho) de 0.219. El coeficiente de endogamia (F) mostró un valor de 0.565 y al evaluar la estructura de la población de estas accesiones, se identificaron 2 grupos (k=2). Un dendograma utilizando “Unweighted Pair Group Method with Arithmetic Mean” (UPGMA), no mostró organización geográfica entre las muestras desconocidas de Puerto Rico. Otro método que se utilizó para evaluar la diversidad genética de papaya en Puerto Rico, fue la identificación de polimorfismos de nucleótido simple (SNPs) utilizando “Genotyping by Sequencing”. Encontramos un total de 4, 245 SNPs. La heterocigosidad observada (Ho) promedio fue 0.226 y el coeficiente de endogamia (Gis) 0.067. La estructura de la población de muestras resultó en 2 grupos. Este estudio contribuye al conocimiento de la diversidad genética de papaya en el Caribe y puede ser útil para la conservación de recursos fitogenéticos.en_US
dc.subjectGenetic diversity of papayaen_US
dc.subjectCarica papayaen_US
dc.subjectPuerto Ricoen_US
dc.subject.lcshPapaya--Puerto Rico--Geneticsen_US
dc.subject.lcshSingle nucleotide polymorphismsen_US
dc.titleMolecular evaluation of the genetic diversity of papaya (Carica papaya) in Puerto Ricoen_US
dc.rights.licenseAll rights reserveden_US
dc.rights.holder(c) 2017 Dianiris Luciano Rosarioen_US
dc.contributor.committeeCuevas, Hugo E.
dc.contributor.committeePorch, Timothy
dc.contributor.committeeRodriguez Minguela, Carlos
dc.contributor.representativeDel Rio, Luis O.
dc.contributor.collegeCollege of Arts and Sciences - Sciencesen_US
dc.contributor.departmentDepartment of Biologyen_US

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