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dc.contributor.advisorMontalvo-Rodríguez, Rafael
dc.contributor.authorSánchez Nieves, Rubén L.
dc.date.accessioned2018-06-06T16:37:27Z
dc.date.available2018-06-06T16:37:27Z
dc.date.issued2017
dc.identifier.urihttps://hdl.handle.net/20.500.11801/730
dc.description.abstractArchaea compose the third domain of life and are most notable for the ability of some to thrive in extreme environments, such environment is that of high salinity. This project focused on the culturable haloarchaeal diversity of the crystallizer ponds of Cabo Rojo, Puerto Rico. Different carbon sources (glycerol vs. pyruvate), water sources (artificial seawater vs crystallizer pond water) , and solidifying agents (agar vs. agarose) combinations were used in media for cultivation. Although no consistent significant difference for colony formation was observed in any combination of variables for the media, members of the genera Haloarcula, Halorubrum, Haloferax, and Halogeometricum were isolated. Phylogenetic analysis utilizing multilocus sequencing analysis was performed to establish relationships with related species. As a result, two novel species of the genera Haloarcula and Halorubrum, respectively, were isolated and subsequently described. The proposed species names are Haloarcula rubripromontorii and Halorubrum tropicale.en_US
dc.description.abstractLas arqueas componen el tercer dominio de la vida y se reconocen principalmente por su habilidad de ser exitosas bajo condiciones extremas. Los ambientes hipersalinos son clasificados como uno de estos lugares extremos. Este projecto se enfocó en la diversidad cultivable de los cristalizadores de Cabo Rojo, PR. Diferentes fuentes de carbono, fuentes (glycerol vs. piruvato) agua (agua salada artificial vs. agua de las salinas) y agentes solidificantes (agar vs. agarosa) fueron utilizadas para los medios de cultivos. Aunque no se encontró alguna ventaja consistente y significativa en conteo de colonias para alguna combinación de variables en el medio, miembros de los géneros Haloarcula, Halorubrim, Haloferax y Halogeometricum fueron aislados y utilizando la técnica de MLSA. Se establecieron relaciones filogenéticas con especies dentro de estos géneros. En fin, dos especies nuevas del género Haloarcula y Halorubrum fueron aisladas y descritas. Los nombres propuestos son Haloarcula rubripromontorii y Halorubrum tropicale.en_US
dc.description.sponsorshipHHMI granten_US
dc.language.isoenen_US
dc.subjectSolar salternsen_US
dc.subjectCabo Rojoen_US
dc.subjectArchaeaen_US
dc.subject.lcshSalt flats--Puerto Rico--Cabo Rojoen_US
dc.subject.lcshArchaebacteria--Molecular aspectsen_US
dc.subject.lcshHalobacteriumen_US
dc.subject.lcshHalophilic microorganisms--Puerto Rico--Cabo Rojoen_US
dc.titleDiversity of the Cabo Rojo solar salterns utilizing culture-dependent methods and description of two novel speciesen_US
dc.rights.licenseAll rights reserveden_US
dc.rights.holder(c) 2017 Rubén L. Sánchez Nievesen_US
dc.contributor.committeePapke, Robertson
dc.contributor.committeeRodríguez-Minguela, Carlos
dc.contributor.committeeSantos-Flores, Carlos J.
dc.contributor.representativeTorres García, Wandaliz
thesis.degree.levelM.S.en_US
thesis.degree.disciplineBiologyen_US
dc.type.thesisThesisen_US
dc.contributor.collegeCollege of Arts and Sciences - Sciencesen_US
dc.contributor.departmentDepartment of Biologyen_US
dc.description.graduationYear2017en_US


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    Items included under this collection are theses, dissertations, and project reports submitted as a requirement for completing a degree at UPR-Mayagüez.

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