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dc.contributor.advisorMassol-Deyá, Arturo A.
dc.contributor.authorToledo-Durán, Gloried E.
dc.date.accessioned2018-08-09T14:18:38Z
dc.date.available2018-08-09T14:18:38Z
dc.date.issued2010
dc.identifier.urihttps://hdl.handle.net/20.500.11801/771
dc.description.abstractThe biogeography of microbial communities in tropical environment is poorly understood. Recent works in soil microbial ecology have focused on cataloging the diversity of soil bacteria, but few of them have documented how soil bacterial communities are affected by a wide range of biotic (fern species) and abiotic (season and study sites, including soil properties) factors.The objective of this work was to analyze biotic and abiotic factors that could affect the biogeography and composition of microbial communities associated with the rhizosphere of two common ferns, Gleichenella pectinata (GP) and Sticherus bifidus (SB) at a copper deposit in Bosque del Pueblo, Barrio Vegas Arriba in Adjuntas (BPA) and a reference site (Bosque Estatal de Maricao, Barrio Tabonuco in Sabana Grande, BEM) during the dry and the wet seasons. The biogeography of rhizosphere-associated microbial communities was evaluated using Terminal Restriction Fragment Length Polymorphisms (TRFLP) and clone libraries. Abiotic factors such as available phosphorus (P), exchangeable calcium (Ca), magnesium (Mg) and potassium (K), organic matter percentage (OM %), total copper (Cu) content and pH were also studied. Data were analyzed using a combination of statistical tools, such as multivariate analysis. Differences in soil microbial community composition were observed and associated with the presence of Firmicutes, Act inobacteria, Proteobacteria, Fusobacteria, Cyanobacteria, Nitrospira, Fibrobacteres and Bacteroidetes. Microbial communities associated with both ferns appeared to be more influence by season, than by fern specie or study site. At BPA, microbial communities from samples collected appeared to be influenced by Cu, P and K while those from BEM were apparently influenced by Ca, Mg, pH taken in H2O and in CaCl2. Microbial communities associated with GP site collected at BEM revealed that their biogeographical distribution during the dry season appeared to be more similar than during the wet season. In contrast, those associated with the SB site seems to have a similar biogeographical distribution during both seasons. The biogeography of microbial communities associated with the GP collected at BPA appeared to be different regardless of the seasons. In contrast, surface communities associated with SB during both seasons and the subsurface communities during the wet season appeared to be similar, but communities at different depths during the dry season were not. This study indicates that different tropical forests within the same geographical region can have different microbial communities due to differences in soil properties, seasons, fern species and study sites.
dc.description.abstractLa biogeografía de comunidades microbianas en ambientes tropicales es pobremente entendida. Trabajos recientes en ecología microbiana del suelo se han enfocado en la clasificación de la diversidad de las bacterias del suelo, pero pocos de ellos han documentado cómo las comunidades bacterianas del suelo son afectadas por una amplia gama de factores bióticos (especie de helechos) y abióticos (época y lugares de muestreo, incluyendo propiedades del suelo). El objetivo de este trabajo fue analizar factores bióticos y abióticos que podían afectar la biogeografía y estructura de las comunidades microbianas asociadas a la rizósfera de dos helechos comunes, Gleichenella pectinata (GP) y Sticherus bifidus (SB), en un depósito de cobre en el Bosque del Pueblo, Bo. Vegas Arriba en Adjuntas (BPA) y en un lugar de referencia (Bosque Estatal de Maricao, Bo. Tabonuco en Sabana Grande, BEM) durante la época seca y la húmeda. La biogeografía de comunidades microbianas asociadas a la rizósfera fue evaluada utilizando polimorfismos de la longitud de los fragmentos terminales de restricción (TRFLP, por sus siglas en inglés) y una biblioteca de clones. Además, factores abióticos como fósforo disponible (P), calcio (Ca), magnesio (Mg) y potasio (K) intercambiable, porcentaje de materia orgánica (OM %), contenido de cobre (Cu) total y pH tomado en H2O y en CaCl2 fueron estudiados. Los datos fueron analizados usando una combinación de herramientas estadísticas, como análisis multivariados. Diferencias en la composicion de comunidades microbianas fueron observadas y asociadas a la presencia de Firmicutes, Actinobacteria, Proteobacteria, Fusobacteria, Cyanobacteria, Nitrospira, Fibrobacteres y Bacteroidetes. Comunidades microbianas asociada a ambos helechos aparentaron estar más influenciadas por la época, que por la especie del helecho o lugar de muestreo. En BPA, las comunidades microbianas de los suelos colectados aparentaron estar influenciadas por Cu, P and K mientras que aquellas del BEM aparentaron estar influenciadas por Ca, Mg, pH tomado en H2O y en CaCl2. Comunidades microbianas asociadas al área de GP colectadas en BEM revelaron que su distribución biogeografíaca durante la época seca aparentó ser más similar que durante la época húmeda. En contraste, aquellas asociadas al área de SB parecieron tener una distribución biogeografía similar durante ambas épocas. La biogeografía de las comunidades microbianas asociadas a GP colectadas en BPA aparentó ser diferente sin importar la época. En contraste, comunidades de la superficie asociadas a SB durante ambas épocas y las comunidades profundas durante la época húmeda aparentaron ser similares, pero aquellas observadas a las distintas profundidades durante la época seca fueron menos similares. Los resultados de este estudio indican que, diferentes bosques dentro de la misma región geográfica pueden tener diferentes comunidades microbianas debido a las especies de helechos presentes, temporadas, lugares de muestreo y propiedades del suelo.
dc.description.sponsorshipDr. Scott Neubauer from the Smithsonian Environmental Research Center Pfizer Pharmaceutical Industry, Quality Control Final Product and Merck Sharp Dohme Quality Operations Casa Pueblo; TMEL USDA-TARSen_US
dc.language.isoenen_US
dc.subjectBiogeography of microbial communitiesen_US
dc.subjectSoil microbial ecologyen_US
dc.subjectDiversity of soil bacteria,en_US
dc.subjectRhizosphereen_US
dc.subjectGleichenella pectinata (GP)en_US
dc.subjectSticherus bifidus (SB)en_US
dc.subject.lcshSoil microbial ecology--Puerto Rico--Adjuntas--Bosque del Puebloen_US
dc.subject.lcshSoil microbial ecology--Puerto Rico--Maricao--Bosque Estatalen_US
dc.subject.lcshSoil microbial ecology--Puerto Rico--Sabana Grandeen_US
dc.subject.lcshBiotic communities--Diversityen_US
dc.subject.lcshBiogeography-- Puerto Ricoen_US
dc.subject.lcshMicrobial diversity--Compositionen_US
dc.titleBiogeography of copper-rich soil microbial communities associated with Gleichenella pectinata and Sticherus bifidus at Bosque del Pueblo (Adjuntas, PR) and Bosque Estatal de Maricao (Sabana Grande, PR)en_US
dc.typeThesisen_US
dc.rights.licenseAll rights reserveden_US
dc.rights.holder(c) 2010 Gloried E. Toledo-Duránen_US
dc.contributor.committeeMontalvo, Rafael
dc.contributor.committeeKolterman, Duane A.
dc.contributor.committeeRodríguez, Carlos
dc.contributor.representativeBeaver, Linda
thesis.degree.levelM.S.en_US
thesis.degree.disciplineBiologyen_US
dc.contributor.collegeCollege of Arts and Sciences - Sciencesen_US
dc.contributor.departmentDepartment of Biologyen_US
dc.description.graduationYear2010en_US


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