Publication:
Infectious capability of the plus (+) and minus (-) strands of parvovirus LUIII

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Authors
De La Mota-Peynado, Alina M.
Embargoed Until
Advisor
Diffoot-Carlo, Nanette
College
College of Arts and Sciences - Sciences
Department
Department of Biology
Degree Level
M.S.
Publisher
Date
2004
Abstract
The replication of autonomous parvovirus LuIII and its encapsidation pattern have not been fully explained. Two genomic clones of LuIII, pBLu(+) and pBLu(-), were constructed. These clones were used to produce the (+) or (-) ss viral DNA, to assess the capability of infection of the individual strands. Three different techniques were utilized to obtain the single-stranded LuIII DNA. Only the treatment with Exonuclease III yielded both strands from the pBLu(+) construct. Each polarity of the single-stranded LuIII DNA was independently transfected into cancerous HeLa cells by electroporation. The results obtained suggest that both the ss(+) and ss(-) LuIII DNA were capable of establishing an infection in these cells. The (-) strand produced 100% cell lysis two days prior to the (+) strand, appearing to be more effective in the establishment of viral infection.

La replicación y el patrón de encapsidación del parvovirus LuIII no han sido explicados completamente. Dos clones genómicos de LuIII, pBLu(+) y pBLu(-), fueron construidos. Estos clones fueron utilizados para tratar de producir ADN viral de hebra sencilla de polaridad (+) o (-). Se intentaron tres técnicas diferentes para obtener el ADN viral de hebra sencilla de LuIII. Solamente el tratamiento con Exonucleasa III produjo ambas hebras utilizando la construcción pBLu(+). Ambas polaridades del ADN de hebra sencilla de LuIII fueron transfectadas a células cancerosas HeLa por electroporación. Los resultados obtenidos sugieren que las hebras de ADN de polaridad (+) y (-) de LuIII fueron capaces de establecer una infección en estas células. La hebra (-) alcanzó 100% de lisis celular dos días antes que la hebra (+), aparentando ser más efectiva en el establecimiento de la infección viral.
Keywords
Replication of autonomous parvovirus LuIII,
Parvovirus LuIII,
Genomic clones of LuIII,
The (+) or (-) ss viral DNA,
Exonuclease III,
Cancerous HeLa cells by electroporation
Cite
De La Mota-Peynado, A. M. (2004). Infectious capability of the plus (+) and minus (-) strands of parvovirus LUIII [Thesis]. Retrieved from https://hdl.handle.net/20.500.11801/801