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dc.contributor.advisorDiffoot-Carlo, Nanette
dc.contributor.authorDe La Mota-Peynado, Alina M.
dc.description.abstractThe replication of autonomous parvovirus LuIII and its encapsidation pattern have not been fully explained. Two genomic clones of LuIII, pBLu(+) and pBLu(-), were constructed. These clones were used to produce the (+) or (-) ss viral DNA, to assess the capability of infection of the individual strands. Three different techniques were utilized to obtain the single-stranded LuIII DNA. Only the treatment with Exonuclease III yielded both strands from the pBLu(+) construct. Each polarity of the single-stranded LuIII DNA was independently transfected into cancerous HeLa cells by electroporation. The results obtained suggest that both the ss(+) and ss(-) LuIII DNA were capable of establishing an infection in these cells. The (-) strand produced 100% cell lysis two days prior to the (+) strand, appearing to be more effective in the establishment of viral infection.en_US
dc.description.abstractLa replicación y el patrón de encapsidación del parvovirus LuIII no han sido explicados completamente. Dos clones genómicos de LuIII, pBLu(+) y pBLu(-), fueron construidos. Estos clones fueron utilizados para tratar de producir ADN viral de hebra sencilla de polaridad (+) o (-). Se intentaron tres técnicas diferentes para obtener el ADN viral de hebra sencilla de LuIII. Solamente el tratamiento con Exonucleasa III produjo ambas hebras utilizando la construcción pBLu(+). Ambas polaridades del ADN de hebra sencilla de LuIII fueron transfectadas a células cancerosas HeLa por electroporación. Los resultados obtenidos sugieren que las hebras de ADN de polaridad (+) y (-) de LuIII fueron capaces de establecer una infección en estas células. La hebra (-) alcanzó 100% de lisis celular dos días antes que la hebra (+), aparentando ser más efectiva en el establecimiento de la infección viral.en_US
dc.subjectReplication of autonomous parvovirus LuIIIen_US
dc.subjectParvovirus LuIIIen_US
dc.subjectGenomic clones of LuIIIen_US
dc.subjectThe (+) or (-) ss viral DNAen_US
dc.subjectExonuclease IIIen_US
dc.subjectCancerous HeLa cells by electroporationen_US
dc.subject.lcshParvovirus infectionsen_US
dc.titleInfectious capability of the plus (+) and minus (-) strands of parvovirus LUIIIen_US
dc.rights.licenseAll rights reserveden_US
dc.rights.holder(c) 2004 Alina M. De La Mota-Peynadoen_US
dc.contributor.committeeVelázquez, Carlos Ríos
dc.contributor.committeeBuxeda, Rosa
dc.contributor.representativeRiquelme, Ernesto
dc.contributor.collegeCollege of Arts and Sciences - Sciencesen_US
dc.contributor.departmentDepartment of Biologyen_US

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    Items included under this collection are theses, dissertations, and project reports submitted as a requirement for completing a graduate degree at UPR-Mayagüez.

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